Anti-AQP3 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q92482

Core Product Specifications and Parameters

Biological Background: Aquaporin-3 Function and Localization

• Aquaporin-3 (AQP3), also known as aquaglyceroporin-3, is a homotetrameric transmembrane channel protein that facilitates the transport of glycerol and water across the plasma membrane driven by osmotic gradients (PubMed:12239222, PubMed:30420639). Related references: PMID:12239222 PMID:30420639
• AQP3 is also permeable to urea and participates in cellular H2O2 permeability, contributing to H2O2-mediated signaling pathways (PubMed:20724658). Related references: PMID:20724658
• In skin, AQP3 transports glycerol to the epidermis and stratum corneum, where it maintains hydration, elasticity, and supports lipid biosynthesis necessary for barrier repair.
• In the kidney, AQP3 is involved in water reabsorption, helping to maintain proper fluid balance.
• The protein localizes to the basolateral membrane of epithelial cells in kidney collecting ducts and airways, and is widely expressed in keratinocytes, immature dendritic cells, and erythrocytes.
• AQP3 is subject to alternative splicing and is classified as a blood group antigen; it is a glycoprotein with multiple transmembrane helices.

Experimental Guidance and Technical Tips

• The immunogen targets amino acids 178–292 of human AQP3 (NP_004916.1), a region that is conserved in mouse and rat, consistent with the reported cross-reactivity.
• For Western blot (WB), a band at approximately 32 kDa is expected; consider using appropriate loading controls and validate in your sample matrix.
• Immunofluorescence (IF-P) and ELISA applications are supported; optimize antibody dilutions and blocking conditions for your specific experimental setup.
• As a polyclonal antibody, batch-to-batch variability may occur; include proper controls and verify performance for critical assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing platform for IVD raw materials, connecting diagnostic manufacturers and research laboratories with high-quality reagents. For aquaporin-3 and related membrane transport research, we supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to support your assay development. From concept to clinic, CamelBio streamlines access to rare and routine targets alike.