Anti-AQP2 Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P41181

Core Product Specifications and Parameters

Biological Background: AQP2 Function and Localization

• AQP2 functions as a water-specific channel, conferring high water permeability to plasma membranes, enabling water transport along osmotic gradients. Related references: PMID:15509592 PMID:7510718 PMID:7524315
• It plays an essential role in renal water homeostasis and is associated with diabetes insipidus. Related references: PMID:15509592 PMID:7524315 PMID:8140421
• AQP2 may also be permeable to glycerol. Related references: PMID:8584435
• The protein localizes to the apical and basolateral cell membranes, as well as cytoplasmic vesicles and the trans-Golgi network membrane.
• It is expressed in the collecting tubules of the kidney medulla.
• Post-translational modifications include glycosylation and phosphorylation.
• Alternative names: Aquaporin-2, ADH water channel, Aquaporin-CD (AQP-CD), Collecting duct water channel protein, WCH-CD.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 147–271), which may require permeabilization for intracellular epitope detection in IF-P and IHC-P; optimize fixation and antigen retrieval conditions.
• For Western blotting, the expected molecular weight is ~29 kDa, though glycosylation may cause a shift; always verify with appropriate molecular weight markers and positive controls.
• Validate the antibody’s reactivity in relevant human, mouse, or rat kidney tissue lysates or cell lines to confirm specificity.
• Consider ELISA protocols with appropriate coating buffers and detection systems for quantitative analysis of AQP2.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with IVD manufacturers and research labs to streamline sourcing of high-quality raw materials like this anti-AQP2 polyclonal antibody, essential for studying renal water transport and diabetes insipidus. We offer a comprehensive portfolio including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and capability to source targets for rare or emerging biomarkers. Whether scaling up production or refining research assays, CamelBio bridges your concept-to-clinic needs with reliable supply solutions.