Anti-AQP2 Polyclonal Antibody for IHC-P, ELISA - P41181

Core Product Specifications and Parameters

Biological Background: AQP2 Function and Localization

• Aquaporin-2 (AQP2) is a water-specific channel protein that facilitates high water permeability across the plasma membranes of renal collecting duct principal cells, playing a critical role in renal water homeostasis (PubMed:15509592, PubMed:7510718, PubMed:7524315, PubMed:8140421). Related references: PMID:15509592 PMID:7510718 PMID:7524315 PMID:8140421
• It belongs to the aquaporin family and is also known as ADH water channel, Aquaporin-CD (AQP-CD), Collecting duct water channel protein, and WCH-CD.
• AQP2 may also exhibit permeability to glycerol, as suggested by functional studies (PubMed:8584435). Related references: PMID:8584435
• Subcellularly, AQP2 is localized to the apical and basolateral plasma membranes, as well as to cytoplasmic vesicle membranes and the trans-Golgi network, reflecting its regulated trafficking in response to vasopressin.
• Expression of AQP2 is predominantly found in the collecting tubules of the kidney medulla, with detection at the protein level in kidney tissue (PubMed:7510718). Related references: PMID:7510718
• Keywords associated with AQP2 include Glycoprotein, Phosphoprotein, and Disease variant, with mutations linked to diabetes insipidus, underscoring its clinical relevance in water balance disorders.

Experimental Guidance and Technical Tips

• Given the immunogen design covering amino acids 147-271, this antibody may recognize the C-terminal region of AQP2. For IHC-P, consider using kidney tissue sections known to express AQP2 in collecting ducts and validate staining with appropriate controls.
• For ELISA, the recombinant immunogen protein can serve as a positive control. Titrate the antibody and optimize detection conditions to achieve the desired sensitivity.
• The antibody exhibits cross-reactivity with mouse, making it suitable for studies in mouse kidney models. Verify performance in your specific application by parallel testing of human and mouse samples.
• Antigen retrieval protocols for formalin-fixed tissues may enhance signal in IHC-P; heat-induced epitope retrieval in citrate buffer (pH 6.0) is a common starting point.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw-material provider, CamelBio supports diagnostic developers and researchers with a wide range of antibodies, including this anti-AQP2 polyclonal antibody for renal and water homeostasis studies. Our portfolio covers validated antibody pairs, bulk monoclonal and polyclonal antibodies, and ancillary reagents, with flexible sourcing options for rare targets. Partner with CamelBio to streamline your procurement from discovery to clinical application.