Anti-APTX Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q7Z2E3

Core Product Specifications and Parameters

Biological Background: APTX Function and Localization

• Aprataxin (APTX), also known as forkhead-associated domain histidine triad-like protein (FHA-HIT), is a DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair (PubMed:15044383, PubMed:15380105, PubMed:16964241). It resolves abortive DNA ligation intermediates by catalyzing the release of adenylate groups from 5'-phosphate termini, generating 5'-phosphate termini that can be efficiently rejoined (PubMed:16964241, PubMed:17276982, PubMed:24362567). Related references: PMID:15044383, PMID:15380105, PMID:16964241, PMID:17276982, PMID:24362567

• APTX also hydrolyzes adenosine 5'-monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), although with lower catalytic activity (PubMed:16547001). It displays higher specific activity with 5'-linked adenosine (AppDNA) compared to 3'-linked guanosine or inosine DNA adducts (By similarity). Related references: PMID:16547001

• The protein localizes predominantly to the nucleus, including the nucleoplasm and nucleolus, but is also detected in the cytoplasm.

• APTX contains a zinc-finger and an FHA domain, requiring metal ion binding for activity. It is subject to phosphorylation (Phosphoprotein keyword).

• Isoform 1 is highly expressed in the cerebral cortex and cerebellum relative to isoform 2. APTX is widely expressed in tissues such as liver, kidney, lymph node, skeletal muscle, fibroblasts, lymphocytes, and pancreas (PubMed:14755728, PubMed:11586299, PubMed:11586300, PubMed:15276230). Related references: PMID:14755728, PMID:11586299, PMID:11586300, PMID:15276230

• Mutations in APTX cause ataxia with oculomotor apraxia type 1 (AOA1), a neurodegenerative disorder, highlighting its essential role in neuronal DNA repair.

Experimental Guidance and Technical Tips

• The immunogen (recombinant protein encompassing amino acids 93-342 of human APTX) covers the catalytic HIT domain; thus, the resulting polyclonal antibody may recognize both isoforms. Verify isoform detection in your sample system.
• For Western blot (WB), the predicted molecular weight is 41 kDa, but post-translational modifications may cause slight shifts. Use appropriate positive controls (e.g., HeLa, HEK293 lysates) and validate with siRNA knockdown if possible.
• In immunofluorescence (IF/ICC), expect nuclear staining with possible nucleolar enrichment. Co-stain with a nucleolar marker (e.g., fibrillarin) to confirm localization.
• For immunoprecipitation (IP), the antibody may capture APTX and its interacting proteins involved in DNA repair. Optimize lysis buffer conditions (e.g., salt concentration, detergent) to preserve protein complexes.
• ELISA applications can be developed using recombinant APTX protein or peptide as a standard. Titrate the antibody for optimal signal-to-noise ratio.
• This antibody is validated for human reactivity; cross-reactivity with other species has not been determined. If using non-human samples, sequence alignment of the immunogen region is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive one-stop sourcing solution for IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Focusing on DNA repair and neurodegenerative disease research, this anti-APTX antibody exemplifies our commitment to supplying high-quality, rare-target raw materials to accelerate your assay development from concept to clinic. Partner with CamelBio for reliable, scalable sourcing and expert technical support tailored to your diagnostic and research needs.