Anti-APRT Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P07741

Core Product Specifications and Parameters

Biological Background: APRT Function and Localization

• APRT (Adenine phosphoribosyltransferase) is the enzyme that catalyzes the formation of AMP from adenine and phosphoribosyl pyrophosphate in the purine salvage pathway, an energetically less costly route than de novo synthesis.
• The protein is a member of the glycosyltransferase family, specifically the pentosyltransferases, and functions as a transferase.
• Alternative splicing generates multiple transcript variants, though the functional significance of different isoforms remains to be clarified.
• APRT localizes primarily to the cytoplasm, consistent with its role in nucleotide metabolism.
• Post-translational modifications include acetylation and phosphorylation, which may regulate enzyme activity or stability.
• According to UniProt, APRT has the keyword 'Disease variant,' indicating that mutations in this gene are associated with human disease.
• The protein has been identified in large-scale proteomics studies and its three-dimensional structure has been solved.
• Calculated molecular weight is approximately 20 kDa (19.6 kDa).

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein covering the full length (aa 1-180) of human APRT. It is expected to recognize both endogenous and overexpressed APRT in human, mouse, and rat samples.
• For Western blot (WB), a band at approximately 20 kDa is anticipated. Optimize loading controls and antibody titration for your specific sample type.
• In immunofluorescence/immunocytochemistry (IF/ICC), non-specific binding may be minimized by using appropriate blocking buffers and secondary antibody controls. Validate in the relevant cell lines.
• For ELISA, consider using the antibody as a capture or detection reagent in indirect or sandwich formats; pairing with a matched detection antibody may improve sensitivity.
• Cross-reactivity with human, mouse, and rat has been indicated; however, epitope conservation analysis is recommended for other species.
• The antibody is supplied as a polyclonal from rabbit, and lot-to-lot consistency should be verified for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-APRT polyclonal antibody supports research into purine metabolism and related disorders; as part of our comprehensive catalog, we also supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can source rare-target raw materials to accelerate your assay development.