Anti-APRT Rabbit Polyclonal Antibody for WB and ELISA - P07741

Core Product Specifications and Parameters

Biological Background: APRT Function and Localization

• APRT encodes adenine phosphoribosyltransferase, an enzyme of the purine salvage pathway. The canonical isoform is approximately 19.6 kDa, and alternative splicing generates additional variants.
• The enzyme catalyzes the conversion of adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP) to AMP and pyrophosphate, a reaction that is energetically less costly than de novo AMP synthesis.
• APRT belongs to the purine/pyrimidine phosphoribosyltransferase family and is critical for recycling adenine from dietary and nucleic acid turnover.
• Subcellular localization is primarily cytoplasmic, consistent with its role in cytoplasmic nucleotide salvage.
• Post-translational modifications include acetylation and phosphorylation, which may modulate enzyme activity or stability.
• Genetic variations in APRT are associated with adenine phosphoribosyltransferase deficiency, a disorder leading to 2,8-dihydroxyadenine urolithiasis and renal failure.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the N-terminal region (aa 1–180) of human APRT, which shares high homology with mouse APRT, supporting the observed cross-reactivity.
• This polyclonal antibody may detect multiple APRT isoforms due to alternative splicing; consider verifying the expected molecular weight (~20 kDa) in your sample system.
• For Western blot, include appropriate negative and positive controls (e.g., APRT-overexpressing lysates or tissues with known expression levels).
• For ELISA, ensure coating compatibility and consider pairing with a secondary detection system optimized for polyclonal rabbit antibodies.
• Given the cytoplasmic localization, mild lysis buffers are recommended for efficient protein extraction.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio provides reliable access to this anti-APRT antibody alongside a wide portfolio of monoclonal and polyclonal antibodies, validated antibody pairs, and ancillary reagents. Whether you are developing diagnostic assays for metabolic disorders or exploring nucleotide salvage pathways, our one-stop sourcing model streamlines procurement from discovery to clinic. Our technical consultants are available to support rare target material sourcing and bulk order customization.