Anti-APRT Rabbit Monoclonal Antibody (KD Validated) for WB, IF/ICC, ELISA - P07741

Core Product Specifications and Parameters

Biological Background: APRT Function and Localization

• Adenine phosphoribosyltransferase (APRT) catalyzes the salvage synthesis of AMP from adenine and 5-phosphoribosyl 1-pyrophosphate (PRPP), a reaction that is energetically less costly than de novo purine biosynthesis.
• This enzyme provides an alternative route to maintain adenylate nucleotide pools, particularly important in tissues with high energy demands or limited de novo synthesis capacity.
• APRT belongs to the purine/pyrimidine phosphoribosyltransferase family and is evolutionarily conserved across species.
• The protein localizes predominantly in the cytoplasm, consistent with its role in cytoplasmic nucleotide metabolism.
• Post-translational modifications include acetylation and phosphorylation, which may regulate enzyme activity or stability; alternative splicing produces multiple transcript variants.
• Mutations in the APRT gene are associated with adenine phosphoribosyltransferase deficiency, a disorder leading to 2,8-dihydroxyadenine urolithiasis and chronic kidney disease (disease variant keyword).
• The molecular weight of the canonical isoform is approximately 19.6 kDa, consistent with the observed 20 kDa band in Western blotting.

Experimental Guidance and Technical Tips

• The immunogen encompasses the full-length human APRT (residues 1–180), suggesting recognition of native and denatured forms; validate in relevant sample systems.
• For Western blot, a band around 20 kDa is expected; use appropriate molecular weight markers and consider loading controls for quantitative analysis.
• In immunofluorescence/immunocytochemistry, cytoplasmic staining should be observed; controls such as APRT knockout cell lines can help confirm specificity.
• ELISA performance may depend on coating conditions and antibody dilution; optimization is recommended.
• Cross-reactivity with mouse and rat APRT has been confirmed, but sequence homology suggests possible recognition of other mammalian species; verify if used beyond human, mouse, or rat.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw material procurement by offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-APRT Rabbit mAb exemplifies our commitment to providing high-quality antibodies for nucleotide metabolism research and diagnostic assay development. Whether you need rare-target antibody sourcing or comprehensive technical support, CamelBio bridges your path from concept to clinic.