Anti-APPL1 Rabbit Polyclonal Antibody for WB, ELISA - Q9UKG1

Core Product Specifications and Parameters

Biological Background: APPL1 Function and Localization

• APPL1 is a multifunctional adapter protein that binds to various membrane receptors, nuclear factors, and signaling proteins to regulate cell proliferation, immune response, endosomal trafficking, and cell metabolism. Related references: PMID:10490823, PMID:15016378, PMID:19661063

• Regulates signaling pathway leading to cell proliferation through interaction with RAB5A and the NuRD/MeCP1 complex. Related references: PMID:15016378

• Plays a role in cell metabolism by regulating adiponectin and insulin signaling pathways. Related references: PMID:19661063, PMID:24879834, PMID:26073777

• Involved in trafficking of the TGFBR1 from the endosomes to the nucleus via microtubules in a TRAF6-dependent manner. Related references: PMID:26583432

• Positive regulator of beta-catenin/TCF-dependent transcription through interaction with RUVBL2/reptin. Related references: PMID:19433865

• Subcellular localization includes early endosome membrane, nucleus, cytoplasm, endosome, cell projection ruffle, and cytoplasmic vesicle, phagosome.

• Tissue specificity: High expression in heart, ovary, pancreas, and skeletal muscle.

• Phosphoprotein; mutations in APPL1 are associated with maturity-onset diabetes of the young type 14 (MODY14).

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein corresponding to the N-terminal region (aa 1-210) of human APPL1; this region may be conserved across species, enabling cross-reactivity with mouse and rat.
• For Western blotting, an expected band size of approximately 80 kDa should be observed; validate using tissues with high APPL1 expression such as heart, ovary, or skeletal muscle.
• For ELISA, consider optimizing coating conditions with the recombinant immunogen or endogenous APPL1 capture, and include appropriate species-matched controls.
• Due to the polyclonal nature, lot-to-lot variability may exist; perform validation for each new lot in the intended application and sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-APPL1 antibody supports research in cell signaling, metabolism, and diabetes; for IVD kit development, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our platform streamlines procurement of rare-target raw materials, helping you accelerate assay development seamlessly.