Anti-APPL1 Rabbit Monoclonal Antibody for WB, ELISA - Q9UKG1

Core Product Specifications and Parameters

Biological Background: APPL1 Function and Localization

• APPL1 is a multifunctional adapter protein that binds to various membrane receptors, nuclear factors, and signaling proteins, regulating essential processes including cell proliferation, immune response, endosomal trafficking, and metabolism. Related references: PMID:10490823, PMID:15016378, PMID:19661063
• Also known as DCC-interacting protein 13-alpha or Adapter protein containing PH domain, PTB domain and leucine zipper motif 1, it acts as a critical node in multiple signaling cascades.
• It positively regulates the innate immune response by forming a complex with PIK3R1 and activating AKT1 signaling.
• APPL1 modulates beta‑catenin/TCF-dependent transcription by directly interacting with RUVBL2/reptin, thereby relieving RUVBL2-mediated repression of target genes. Related references: PMID:19433865
• The protein localizes to the early endosome membrane, nucleus, cytoplasm, cell ruffles, and phagosomes, reflecting its dynamic role in intracellular trafficking and signaling.
• It is highly expressed in heart, ovary, pancreas, and skeletal muscle, consistent with its functions in metabolic and cardiovascular tissues.
• Key post‑translational and structural features include phosphorylation and a coiled‑coil domain, with reported involvement in cell cycle, diabetes mellitus, and disease.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 350–450 of human APPL1; consider verifying antibody specificity against the full‑length protein in relevant lysates by Western blot.
• As the antibody is validated for WB and ELISA, appropriate blocking and dilution conditions should be optimized for each application and sample type.
• It cross‑reacts with mouse and rat APPL1, enabling cross‑species comparative studies; however, we recommend confirming reactivity in the chosen experimental system.
• The predicted molecular weight is ~80 kDa; use a suitable molecular weight marker and ensure complete transfer in WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting. This anti‑APPL1 antibody is just one example of our extensive line of validated monoclonal antibodies for cell signaling and metabolism research. In addition to ready‑to‑use antibodies, we offer bulk ancillary reagents and custom sourcing for rare targets, helping you accelerate your projects from concept to clinic.