Anti-APPBP2 Rabbit Polyclonal Antibody for WB, ELISA - Q92624

Core Product Specifications and Parameters

Biological Background: APPBP2 Function and Localization

• APPBP2 (Amyloid protein-binding protein 2, also known as APP-BP2 or PAT1) is a substrate-recognition component of the Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex in the DesCEND pathway. Related references: PMID:29775578, PMID:29779948, PMID:37844242
• It specifically targets proteins possessing a C-terminal -Arg-Xaa-Xaa-Gly degron motif, leading to their ubiquitination and subsequent proteasomal degradation. Related references: PMID:29775578, PMID:29779948, PMID:37844242
• Functions include mediating degradation of truncated proteins, such as SELENOV selenoproteins produced by failed UGA/Sec decoding, and negatively regulating beige adipocyte differentiation by degrading PRDM16. Related references: PMID:26138980, PMID:37844242
• May also participate in intracellular protein transport, facilitating the translocation of amyloid precursor protein (APP) along microtubules to the cell surface. Related references: PMID:9843960
• Localizes to the nucleus, cytoplasm, cytoskeleton, and membrane compartments.
• Belongs to the proteomics identification and reference proteome datasets, and features TPR repeat domains critical for protein–protein interactions.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 386–585) of human APPBP2, which is well conserved across human, mouse, and rat; this supports the antibody’s cross-reactivity profile.
• For Western blotting, consider using whole cell lysates from human, mouse, or rat tissues/cell lines known to express APPBP2, and expect a band at approximately 67 kDa.
• In ELISA applications, the antibody may be used for direct antigen detection or as a capture/detection reagent; we recommend initial titration experiments to determine optimal working concentrations.
• As a polyclonal antibody, verify lot-to-lot consistency in your specific assay system and include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing solution for IVD raw materials, including validated polyclonal antibodies like the APPBP2 Rabbit pAb. Ideal for research into neurodegenerative diseases and ubiquitin-mediated proteolysis, we supply bulk ancillary reagents and can assist with custom antibody generation. Let CamelBio be your trusted partner from discovery to diagnostic application.