Anti-APP Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P05067

Core Product Specifications and Parameters

Biological Background: APP Function and Localization

• APP (Amyloid-beta precursor protein) is a type I transmembrane protein that undergoes alternative splicing to generate several isoforms, including APP695, APP751, and APP770, with APP695 predominating in neurons.
• Acts as a cell surface receptor on neurons, where homophilic interaction between APP molecules on neighboring cells promotes synaptogenesis. Related references: PMID:25122912
• Functions as a kinesin I membrane receptor, mediating the axonal anterograde transport of beta-secretase and presenilin 1 toward synapses. Related references: PMID:17062754 PMID:23011729
• Proteolytic processing yields amyloid-beta peptides, which are lipophilic metal chelators implicated in Alzheimer's disease pathology and can induce oxidative stress and neurotoxicity.
• Localizes to multiple compartments, including the cell membrane, growth cones, early endosomes, endoplasmic reticulum, Golgi apparatus, and can be secreted; it also appears in the nucleus and cytoplasm.
• Exhibits a wide tissue expression profile, with high levels in brain, kidney, heart, and spleen; in adult brain, frontal lobe and perisylvian cortex‑opercular gyri show the strongest expression.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider a dilution range of 1:500–1:2000 and validate the expected band near 87 kDa in brain lysates from human, mouse, or rat. Use protease inhibitors to limit APP degradation.
• For immunofluorescence/immunocytochemistry (IF/ICC), optimize a starting dilution of 1:50–1:200 and permeabilize cells to detect intracellular APP pools. Include appropriate controls to confirm staining specificity.
• For ELISA, this polyclonal antibody may be used as capture or detection reagent; cross‑check performance with recombinant APP peptides and validate in relevant sample matrices such as serum or CSF.
• The immunogen (N‑terminal residues 1‑100) is conserved across major isoforms, so the antibody may recognize multiple APP species and some N‑terminal fragments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one‑stop IVD raw‑material sourcing partner, offering validated antibody pairs, optimized polyclonal antibodies like this APP Rabbit pAb, and bulk ancillary reagents to advance Alzheimer's disease research and diagnostic development. Leveraging expertise in rare‑target sourcing, we streamline your path from concept to clinic. For your APP‑related projects, count on our comprehensive supply chain to deliver high‑quality reagents and technical support efficiently.