Anti-ApoM Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O95445

Core Product Specifications and Parameters

Biological Background: Apolipoprotein M Function and Localization

• Apolipoprotein M (ApoM) is a plasma protein primarily expressed in the liver and kidney, and is also known as Protein G3a.
• It is a secreted protein associated with high-density lipoprotein (HDL) and plays a probable role in lipid transport.
• ApoM can bind a variety of hydrophobic ligands, including sphingosine-1-phosphate, myristic acid, palmitic acid, stearic acid, retinol, all-trans-retinoic acid, and 9-cis-retinoic acid, suggesting a function in the distribution of these molecules.
• The gene undergoes alternative splicing, as indicated by the 'Alternative splicing' keyword, though specific isoforms beyond the canonical sequence are not detailed here.
• As a component of HDL particles, ApoM contributes to the structural integrity and functional repertoire of these lipoprotein complexes.
• Post-translational modifications include glycosylation (as noted by the 'Glycoprotein' keyword) and the presence of disulfide bonds, which may be important for its proper folding and stability.
• Its presence in the secreted proteome and identification by mass spectrometry-based proteomics confirm its extracellular localization and facilitate biomarker discovery efforts.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 22–188 of human ApoM, representing a large portion of the mature protein; this design supports recognition of the native secreted form in applications such as ELISA and IF/ICC.
• The antibody is indicated for WB, IHC-P, IF/ICC, and ELISA. For IHC-P, consider validating in human liver or kidney sections where ApoM is highly expressed.
• Cross-reactivity is reported for human, mouse, and rat. For rodent studies, verify specificity in the relevant tissue or cell models, as sequence identity in the immunogen region may vary slightly.
• The predicted molecular weight of the unmodified protein is 21.253 kDa, but the product sheet lists 21 kDa; glycosylation may cause a shift in apparent molecular weight on SDS-PAGE. Consider using appropriate positive controls and optimizing loading conditions.
• When using for WB, reduced samples may show a band near 21 kDa; however, disulfide bonds might influence migration under non-reducing conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio equips diagnostic manufacturers, research laboratories, and academic institutes with integrated access to IVD raw materials and technical support, streamlining the journey from early-stage concept to clinical application. This ApoM polyclonal antibody exemplifies our curated portfolio for lipid metabolism and HDL research, and we extend our supply capabilities to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing of rare-target raw materials. Contact us to discuss how our end-to-end procurement solutions can accelerate your biomarker and diagnostic development programs.