Anti-Apolipoprotein M (ApoM) Rabbit Monoclonal Antibody for ELISA, IF/ICC - O95445

Core Product Specifications and Parameters

Biological Background: Apolipoprotein M Function and Localization

• Apolipoprotein M (ApoM) is a secreted glycoprotein belonging to the lipocalin superfamily, primarily associated with high-density lipoprotein (HDL) particles.
• It is probably involved in lipid transport, with the ability to bind sphingosine-1-phosphate, myristic acid, palmitic acid, stearic acid, retinol, all-trans-retinoic acid, and 9-cis-retinoic acid.
• ApoM is predominantly expressed in liver and kidney and circulates as a plasma protein, playing a key role in HDL remodeling and functionality.
• Subcellularly, ApoM is secreted into the extracellular space following cleavage of its signal peptide, consistent with its role as a carrier protein.
• Post-translational modifications include a conserved disulfide bond and N-linked glycosylation, which may influence protein stability and HDL binding.
• Alternative splicing generates multiple transcript variants, though the functional significance of these isoforms remains to be fully characterized.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–100), so consider using this antibody for detecting both full-length and potentially cleaved forms of ApoM.
• For immunofluorescence applications (IF/ICC, IF-P), validate the staining pattern in relevant cell types or tissue sections, as ApoM is secreted and may require fixation optimization to retain extracellular localization.
• In ELISA, pre-coating with the immunogen peptide or recombinant ApoM may help establish a standard curve and assess antibody specificity.
• As a rabbit monoclonal, this reagent offers consistent lot-to-lot performance; nevertheless, titrate the antibody in your specific assay system to determine the optimal working concentration.

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