Anti-Apolipoprotein E (Apoe) Rabbit Polyclonal Antibody for WB - P08226

Core Product Specifications and Parameters

Biological Background: Apolipoprotein E Function and Localization

• Apolipoprotein E (Apoe) is a core component of plasma lipoproteins, playing a central role in lipid transport between organs via plasma and interstitial fluids.
• Apoe associates with chylomicrons, chylomicron remnants, VLDL, IDL, and shows preferential binding to HDL, facilitating their production, conversion, and clearance.
• It mediates cellular uptake of lipoprotein particles by binding to LDL receptor (LDLR) and VLDL receptor (VLDLR) (By similarity).
• Apoe possesses heparin-binding activity, interacting with heparan-sulfate proteoglycans on cell surfaces to support capture and receptor-mediated uptake of lipoproteins (PubMed:23676495) Related references: PMID:23676495
• Subcellularly, Apoe is secreted and found in extracellular space, extracellular matrix, and associated with extracellular vesicles; it is also localized to endosomes and multivesicular bodies.
• The protein undergoes post-translational modifications including glycosylation, phosphorylation, and oxidation, as inferred from UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20-200 of mouse Apoe, which includes the receptor-binding region; this sequence is conserved in rat, supporting the observed cross-reactivity.
• For Western blot (WB), a band at approximately 36 kDa is expected; consider using a relevant positive control such as mouse liver or plasma.
• In immunohistochemical (IHC) staining, Apoe is typically detected in hepatocytes and macrophages; antigen retrieval methods may be required for paraffin-embedded sections.
• For ELISA applications, the polyclonal nature of this antibody allows capture/detection pair possibilities; pre-validation in the specific assay system is recommended.
• The antibody is also suitable for immunofluorescence (IF); fixative conditions should be optimized to preserve antigenicity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and laboratories a streamlined sourcing solution for IVD raw materials. Our portfolio includes validated polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. This anti-Apoe antibody supports lipid metabolism research and cardiovascular disease diagnostics, and we can provide complementary reagents to accelerate your assay development.