Anti-Apolipoprotein B (ApoB) Monoclonal Antibody for WB and ELISA - P04114

Core Product Specifications and Parameters

Biological Background: Apolipoprotein B Function and Localization

• ApoB exists in two main isoforms: ApoB-100 (full-length, 516 kDa) and ApoB-48 (truncated, 48% of N-terminus), produced via RNA editing in the intestine.
• ApoB-100 is a major protein component of very low-density lipoproteins (VLDL) and low-density lipoproteins (LDL), while ApoB-48 is found in chylomicrons.
• ApoB-100 serves as the ligand for the LDL receptor (apoB/E receptor), mediating cellular uptake and clearance of LDL cholesterol.
• Subcellularly, ApoB is located in the cytoplasm, secreted into the bloodstream, and associates with lipid droplets during lipoprotein assembly.
• The protein undergoes extensive post-translational modifications including acetylation, phosphorylation, palmitoylation, and glycosylation, and contains critical disulfide bonds.
• Genetic variants in APOB are associated with atherosclerosis, familial hypercholesterolemia, and other lipid disorders.
• Keywords reflecting its biological role: cholesterol metabolism, lipid transport, heparin-binding, and disease involvement.

Experimental Guidance and Technical Tips

• The immunogen (residues 50-150) is present in both ApoB-100 and ApoB-48; thus the antibody may detect both isoforms in relevant samples.
• For Western blot, use low-percentage polyacrylamide gels (e.g., 4–6%) and extended transfer times to accommodate the high molecular weight (516 kDa) of ApoB-100.
• Validate specificity with appropriate positive (e.g., human liver or plasma) and negative controls, and confirm cross-reactivity with rat samples as indicated.
• In ELISA applications, optimize antibody concentration for capture or detection roles using recombinant ApoB or purified LDL as the antigen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, including this validated anti-ApoB monoclonal antibody, supporting cardiovascular biomarker research and diagnostic assay development. We supply optimized antibody pairs, bulk quantities, and ancillary reagents, and can assist in sourcing rare-target raw materials to streamline your development from concept to clinic.