Anti-Apolipoprotein A2 Rabbit Monoclonal Antibody for WB, IF-P, ELISA - P02652

Core Product Specifications and Parameters

Biological Background: Apolipoprotein A2 Function and Localization

• Apolipoprotein A2 (apoA-II, ApoA2) is a component of high-density lipoprotein (HDL) particles, synthesized primarily in the liver and intestine, and secreted into the plasma.
• It is encoded by the APOA2 gene in humans, and the mature protein is derived from a precursor by cleavage of a signal peptide and a propeptide.
• The primary function of apoA-II is to stabilize HDL structure through lipid association, influencing HDL metabolism and lipid transport.
• Subcellular localization is exclusively extracellular (secreted), consistent with its role in plasma lipoprotein particles.
• Post-translational modifications include disulfide bond formation (homodimerization), glycosylation, oxidation, and phosphorylation; the N-terminal glutamine can form pyrrolidone carboxylic acid.
• The protein is involved in host-virus interactions, as annotated in UniProt, though specific mechanisms remain unclear.
• Tissue specificity: present in plasma; expression is restricted to the liver and intestine under normal conditions.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length mature protein (amino acids 1–110 of NP_001634.1), suggesting the antibody may detect both precursor and processed forms.
• For Western blot (WB), the predicted molecular weight is ~17 kDa under reducing conditions, but dimerization may result in bands at ~34 kDa; validate in your sample system.
• For immunofluorescence (IF-P), consider optimal fixation and permeabilization methods, as the target is secreted and may require detection in tissue sections where it accumulates.
• ELISA applications require standard optimization; pairing with a suitable capture antibody may enhance sensitivity.
• Cross-reactivity with mouse and rat has been reported, but sequence alignment should be checked if using other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing solution for IVD raw materials, supporting manufacturers, laboratories, and research institutes from concept to clinic. For Apolipoprotein A2 research in lipid metabolism and cardiovascular diseases, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development and production workflows.