Anti-Apolipoprotein A2 Polyclonal Antibody for WB, IF/ICC, ELISA - P02652

Core Product Specifications and Parameters

Biological Background: Apolipoprotein A2 Function and Localization

• Apolipoprotein A-II (ApoA-II) is a major protein constituent of high-density lipoprotein (HDL) particles.
• Encoded by the APOA2 gene in humans, it is primarily synthesized in the liver and intestine and secreted into plasma.
• The protein associates with lipids to stabilize HDL structure and may influence HDL metabolism and reverse cholesterol transport.
• Contains a signal peptide for secretion and features a disulfide bond linking two monomers into a homodimer.
• Post-translational modifications include glycosylation, phosphorylation, and oxidation, and it undergoes cleavage at basic residues.
• Keywords indicate involvement in host-virus interactions, suggesting a role in viral entry or immune response.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1–100 of human ApoA-II (NP_001634.1). For Western blot, the theoretical monomeric mass is ~11 kDa, but the mature secreted protein often forms ~22 kDa disulfide-linked dimers or migrates at higher apparent masses due to glycosylation. Validate observed bands in your specific sample and conditions.
• For immunofluorescence/immunocytochemistry, note that ApoA-II is a secreted protein; intracellular detection may require permeabilization and careful fixation. Use liver- or intestine-derived cell lines (e.g., HepG2) or tissue sections as positive controls.
• In ELISA applications, consider using this polyclonal antibody as either capture or detection reagent; optimal pairing with a monoclonal antibody targeting a non-overlapping epitope could enhance assay specificity, but independent validation is recommended.
• As a rabbit polyclonal antibody, background may vary across applications; optimize blocking conditions and include appropriate negative controls.

CamelBio: Your One-Stop Sourcing Bridge

The Apolipoprotein A2 antibody supports research into HDL metabolism, lipid transport, and cardiovascular disease. CamelBio serves as a comprehensive IVD raw-material partner, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline diagnostic development from concept to clinic.