Anti-Apolipoprotein A1/APOA1 Monoclonal Antibody for WB, IF/ICC, ELISA - P02647

Core Product Specifications and Parameters

Biological Background: Apolipoprotein A1/APOA1 Function and Localization

• Apolipoprotein A-I (APOA1) is the major protein component of high-density lipoprotein (HDL) in plasma.
• It plays a key role in reverse cholesterol transport, promoting cholesterol efflux from tissues and acting as a cofactor for lecithin cholesterol acyltransferase (LCAT).
• As part of the SPAP complex, APOA1 is involved in activating spermatozoa motility.
• APOA1 is synthesized in the liver and small intestine and is secreted into the bloodstream.
• It is also found in chylomicrons and is critical for lipid metabolism and transport.
• Oxidation of Met-110 and Met-136 is associated with increased coronary artery disease risk, such as in individuals with the eNOSa/b genotype or exposure to cigarette smoking.
• Elevated levels of oxidized APOA1 are observed in aortic lesions and correlate with disease severity.
• Keywords: Secreted, HDL, cholesterol metabolism, lipid transport, atherosclerosis, amyloidosis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature APOA1 protein (residues 25-267), suggesting the antibody likely recognizes the processed, secreted form.
• For Western blot (WB), the predicted molecular weight of the mature protein is approximately 28 kDa; however, observed bands may vary due to post-translational modifications such as glycosylation or oxidation.
• When performing IF/ICC, consider examining APOA1 localization in liver or intestinal cell lines, or in macrophage foam cells for atherosclerosis studies.
• For ELISA, validate the antibody in the appropriate biological matrix (e.g., plasma or serum) where APOA1 is abundant, and consider the potential for cross-reactivity with oxidized forms.
• Since the antibody cross-reacts with mouse and rat, it can be used for translational models; however, verify performance in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-APOA1 monoclonal antibody supports research into cholesterol metabolism, atherosclerosis, and cardiovascular disease, aligning with CamelBio's commitment to enabling diagnostic development from concept to clinic.