Anti-APOL1 Polyclonal Antibody for IHC-P, ELISA - O14791

Core Product Specifications and Parameters

Biological Background: APOL1 Function and Localization

• Apolipoprotein L1 (APOL1), also referred to as Apo-L, ApoL, or Apolipoprotein L-I, is a secreted glycoprotein belonging to the apolipoprotein L family.
• It may participate in lipid exchange and transport, including reverse cholesterol transport from peripheral cells to the liver, thereby contributing to systemic cholesterol homeostasis.
• APOL1 is a key component of trypanosome lytic factor (TLF) in human serum, conferring innate immunity against Trypanosoma brucei. It forms pores in parasite lysosomal membranes and sensitizes the pathogen to oxidation-driven osmotic lysis.
  Related references:
  PMID:12621437, PMID:16020735, PMID:19997494
• The protein is found on high-density lipoprotein (HDL3) particles in plasma and is broadly expressed in tissues such as pancreas, lung, prostate, liver, placenta, and spleen.
• Post-translational modifications include N-linked glycosylation and phosphorylation, and the gene undergoes alternative splicing, yielding multiple isoforms. Genetic variants in APOL1 are associated with kidney disease, including focal segmental glomerulosclerosis (FSGS4).
• Keywords associated with APOL1 encompass lipid/cholesterol/steroid metabolism, HDL, transport, and disease variant, underscoring its dual roles in lipid biology and innate immunity.

Experimental Guidance and Technical Tips

• For IHC-P, consider testing citric acid or EDTA-based antigen retrieval on formalin-fixed paraffin-embedded sections; rat tissue may serve as a useful positive control given the antibody's cross-reactivity.
• In ELISA, pairing with a matched detection antibody and validating with recombinant APOL1 (aa 179–398) can help establish assay linearity and sensitivity.
• Because APOL1 is a secreted lipoprotein, ensure appropriate sample preparation (e.g., delipidation or albumin depletion) when analyzing plasma or serum samples.
• Always run negative controls (e.g., secondary antibody alone) to confirm signal specificity, and titrate the antibody to optimize signal-to-noise ratios in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting research in lipid metabolism, kidney disease, and infectious diseases. Our catalog includes validated anti-APOL1 antibodies and complementary reagents, while our technical team can assist in sourcing rare-target raw materials and developing custom solutions to streamline your assay development from concept to clinic.