Anti-APOH Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P02749

Core Product Specifications and Parameters

Biological Background: APOH Function and Localization

• Beta-2-glycoprotein 1 (APOH) is also referred to as APC inhibitor, activated protein C-binding protein, anticardiolipin cofactor, and apolipoprotein H (Apo-H).
• It binds to negatively charged substances such as heparin, phospholipids, and dextran sulfate, potentially preventing activation of the intrinsic blood coagulation cascade by binding to damaged cell surfaces.
• The protein is secreted and primarily expressed by the liver, circulating in plasma.
• Structural features include disulfide bonds, glycosylation, and sushi repeat domains, classifying it as a glycoprotein and a member of the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 21-345, covering the mature protein sequence, which may enhance recognition of the native form; validate detection in your target sample system.
• For Western blotting, consider standard reducing conditions; the predicted molecular weight is 38 kDa, but glycosylation may shift the observed band.
• Cross-reactivity with mouse protein suggests conserved epitopes; optimize antibody dilution for each application (WB, IF/ICC, ELISA) using appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop supply of IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support coagulation and lipid metabolism research. For this anti-APOH antibody or rare target sourcing, we deliver reliable procurement and technical support, streamlining your journey from concept to diagnostic application.