Anti-ApoER2/LRP8 Rabbit Monoclonal Antibody for WB, ELISA - Q14114

Core Product Specifications and Parameters

Biological Background: ApoER2/LRP8 Function and Localization

• Serves as a cell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands, mediating the extracellular Reelin signal to intracellular pathways by binding DAB1 on its cytoplasmic tail. Related references: PMID:12899622, PMID:12950167, PMID:20223215
• Key component of the Reelin pathway, which governs neuronal layering of the forebrain during embryonic brain development; exhibits higher affinity for Reelin than the related VLDL receptor (VLDLR).
• Binds dimers of beta 2-glycoprotein I and may be involved in the suppression of platelet aggregation in the vasculature. Related references: PMID:12807892
• Highly expressed in the initial segment of the epididymis, where it influences the functional expression of clusterin and phospholipid hydroperoxide glutathione peroxidase (PHGPx), two proteins required for sperm maturation.
• Also acts as a receptor for Semliki Forest virus and tick-borne encephalitis virus, mediating viral cell attachment and internalization.
• Localizes to the cell membrane and can be secreted; expressed mainly in brain and placenta, as well as in platelets and megakaryocytic cells, but not in the liver.
• Contains multiple domains including EGF-like domains, LDL-receptor class A repeats, and a transmembrane helix; undergoes alternative splicing and is subject to ubiquitination and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 864–963 within the cytoplasmic domain of human LRP8. For Western blotting, consider using cell or tissue lysates from brain, placenta, or platelets as positive controls. The predicted molecular weight is ~106 kDa, but additional bands may appear due to glycosylation or alternative splicing.
• For ELISA, pairing this detection antibody with a capture reagent that recognizes an extracellular epitope may be effective. Validate working concentrations in the relevant sample matrix.
• This antibody is indicated for use in WB and ELISA; cross-reactivity with mouse and rat orthologs suggests utility in rodent model studies, but confirm performance under your specific conditions.

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CamelBio supports diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. For neurodevelopment and infectious disease research programs, we offer one-stop access to critical tools such as this anti-LRP8 monoclonal antibody, ensuring streamlined procurement from concept to clinic.