Anti-Apoe Rabbit Polyclonal Antibody for WB and ELISA - P02649

Core Product Specifications and Parameters

Biological Background: Apolipoprotein E Function and Localization

• APOE is a core component of plasma lipoproteins, mediating lipid transport between organs via association with chylomicrons, VLDL, IDL, and HDL. Related references: PMID:14754908 PMID:1911868 PMID:6860692 PMID:2762297 PMID:9395455
• APOE binds the LDL receptor, LRP1, LRP2, LRP8, and VLDLR to facilitate cellular uptake of lipoproteins. It also interacts with heparan sulfate proteoglycans on cell surfaces, enhancing lipoprotein capture. Related references: PMID:12950167 PMID:1530612 PMID:1917954 PMID:23676495 PMID:7635945 PMID:9395455
• A main function of APOE is to mediate hepatic clearance of chylomicrons, VLDLs, and HDLs, critically maintaining plasma lipid homeostasis and delivering triglycerides to peripheral tissues. Related references: PMID:1911868 PMID:1917954 PMID:23676495 PMID:2762297 PMID:29516132
• APOE participates in reverse cholesterol transport: it cooperates with ABCA1 for HDL biogenesis in tissues and enriches in circulating HDLs for hepatocyte uptake, thus regulating cholesterol homeostasis. Related references: PMID:14754908 PMID:23620513 PMID:9395455
• In the central nervous system, APOE transports lipids and supports neuron survival and sprouting, with high expression in astrocytes and neurons of the cerebral cortex and hippocampus. Related references: PMID:25173806 PMID:8939961 PMID:10027417 PMID:3115992
• APOE modulates innate and adaptive immunity; it binds the immune checkpoint receptor LILRB4, influencing myeloid-derived suppressor cell survival. Related references: PMID:30333625
• APOE interacts with HCV envelope glycoprotein E2, promoting viral attachment to host cells and contributing to the assembly and infectivity of viral particles. Related references: PMID:25122793 PMID:29695434

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 211–311 of mouse Apoe, a region highly conserved in human, which may underlie the observed cross-reactivity. When performing Western blot, consider using standard blocking agents and detecting a band near 36 kDa; validate with a suitable loading control.
• For ELISA applications, it is advisable to titrate the antibody and confirm specificity using recombinant protein or lysates from Apoe-expressing cells as positive controls, and Apoe-knockout or knockdown samples as negative controls.
• As a polyclonal antibody, batch-to-batch variability is possible; we recommend optimizing the dilution for each new lot and application. Include an appropriate secondary antibody control to distinguish specific signals from background.
• To preserve activity, store aliquots at recommended temperatures and avoid repeated freeze‑thaw cycles, as indicated by ice bag shipping.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, CROs, and research laboratories with a comprehensive portfolio of IVD raw materials. This anti-Apoe polyclonal antibody exemplifies our commitment to supporting lipid metabolism, cardiovascular, and neurodegenerative disease research. Beyond individual antibodies, CamelBio offers validated antibody pairs, bulk ancillary reagents, and sourcing for rare target raw materials—providing a seamless supply chain from target discovery to diagnostic development.