Anti-APOC1 Rabbit Polyclonal Antibody for IHC-P and ELISA - P02654

Core Product Specifications and Parameters

Biological Background: APOC1 Function and Localization

• Apolipoprotein C-I (APOC1) is a small secreted protein that primarily associates with high-density lipoproteins (HDL) and triglyceride-rich lipoproteins in plasma, constituting approximately 10% of VLDL and 2% of HDL protein.
• APOC1 functions as an inhibitor of lipoprotein binding to the low-density lipoprotein (LDL) receptor, LDL receptor-related protein (LRP), and very low-density lipoprotein (VLDL) receptor, modulating lipid uptake and metabolism.
• It serves as the major plasma inhibitor of cholesteryl ester transfer protein (CETP), thereby influencing cholesterol ester transfer between lipoproteins.
• APOC1 directly binds free fatty acids, reducing their intracellular esterification and interfering with cellular fatty acid uptake.
• The protein modulates the interaction of apolipoprotein E (APOE) with beta-migrating VLDL and inhibits the binding of beta-VLDL to the LDL receptor-related protein, affecting remnant lipoprotein clearance.
• APOC1 is synthesized mainly in the liver, with minor production in the intestine, and is also detected in the lung and spleen.
• According to UniProt, the theoretical molecular weight is 9332 Da; the gene is located on chromosome 19 and belongs to the apolipoprotein gene family.

Experimental Guidance and Technical Tips

• Given the immunogen spans the full-length mature protein (amino acids 1-83), the antibody is expected to recognize both native and denatured forms; however, validation under your specific experimental conditions is recommended.
• For IHC-P applications, consider standard antigen retrieval techniques (e.g., citrate buffer, pH 6.0) and include liver tissue as a positive control; the antibody has confirmed cross-reactivity with rat.
• In ELISA, the antibody can be used for detection of APOC1; titrate the antibody to determine optimal working concentration and validate using recombinant protein or known positive samples.
• As a polyclonal antibody, lot-to-lot variability may occur; we advise performing appropriate negative controls (e.g., tissue from APOC1 knockout or blocking peptide) to confirm specificity.
• Although not tested by the supplier, users may evaluate performance in other applications (e.g., Western blot) by confirming reactivity with the 9 kDa band; note that the antibody might recognize multiple isoforms or post-translationally modified forms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw material sourcing, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and support for rare target development. This APOC1 rabbit polyclonal antibody serves as a reliable tool for cardiovascular and lipid metabolism research, enabling diagnostic assay development and biomarker studies. Our team is dedicated to bridging the gap from concept to clinic with comprehensive technical services and a robust supply chain.