Anti-APOBEC3G Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9HC16

Core Product Specifications and Parameters

Biological Background: APOBEC3G Function and Localization

• APOBEC3G (A3G/ARCD/ARP-9/CEM15) is a cytidine deaminase that inhibits retrovirus replication and retrotransposon mobility through both deaminase-dependent and independent mechanisms. Related references: PMID:12808465, PMID:16527742, PMID:17121840

• It exhibits potent antiviral activity against Vif‑deficient HIV‑1, acting after viral nucleocapsid penetration by deaminating cytosine to uracil in minus‑strand viral DNA, resulting in G‑to‑A hypermutations that impair the proviral genome. Related references: PMID:12167863, PMID:12859895, PMID:14557625, PMID:12808465, PMID:12808466, PMID:12809610

• APOBEC3G selectively targets single‑stranded DNA and does not deaminate double‑stranded DNA or RNA. Related references: PMID:12808465, PMID:12809610, PMID:12970355

• In addition to HIV‑1, it restricts a broad range of viruses including SIVs, HBV, EIAV, XMRV, and SFV. Related references: PMID:15031497, PMID:16378963, PMID:18448976

• APOBEC3G localizes predominantly to the cytoplasm and P‑bodies, but can also be found in the nucleus; its cytoplasmic retention is linked to its antiviral function.

• Tissue expression is observed in spleen, testes, ovary, peripheral blood leukocytes and CD4+ lymphocytes, and is strongly modulated by the activation/differentiation state of target cells, which correlates with HIV‑1 susceptibility.

• The protein contains two zinc‑coordinating deaminase domains, undergoes phosphorylation, ubiquitination, and other post‑translational modifications that regulate its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 244–257) lies within a conserved region of human APOBEC3G, which may explain the reported cross‑reactivity with mouse and rat orthologs.
• For western blotting (WB), the predicted molecular weight is approximately 46 kDa; however, post‑translational modifications or sample-specific factors may lead to the appearance of additional bands. Validate antibody specificity with appropriate positive and negative control lysates.
• For IHC-P and IF/ICC, optimize antigen retrieval methods and antibody dilutions for the specific tissue or cell type. Consider using HIV‑1‑permissive vs. non‑permissive cell lines as controls to confirm functional relevance.
• In ELISA applications, paired detection with a capture antibody may enhance assay sensitivity; titrate the antibody to determine optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw‑material partner serving diagnostic manufacturers, research institutes, and clinical laboratories worldwide. This anti‑APOBEC3G polyclonal antibody enables robust detection of a central innate immunity restriction factor, supporting studies in antiviral defense, HIV‑1 host‑pathogen interactions, and cancer biology. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and custom sourcing for rare targets—providing a seamless procurement experience from concept to clinical application.