Anti-APOBEC3G Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9HC16

Core Product Specifications and Parameters

Biological Background: APOBEC3G Function and Localization

• APOBEC3G (A3G) is a DNA dC->dU-editing enzyme belonging to the APOBEC3 family of cytidine deaminases. It exists in multiple isoforms due to alternative splicing.
• It acts as a potent restriction factor against Vif-deficient HIV-1 by deaminating cytidine to uridine in single-stranded viral DNA, causing G-to-A hypermutations in the proviral genome. Related references: PMID:12808465, PMID:12808466, PMID:12809610
• The antiviral activity extends to other retroviruses including SIV, HBV, EIAV, XMRV, and SFV, and also inhibits LTR and non-LTR retrotransposon mobility. Related references: PMID:15031497, PMID:16378963, PMID:18448976, PMID:16527742
• A deamination-independent mechanism contributes to antiretroviral effects before proviral integration. Related references: PMID:12808465, PMID:12808466, PMID:12809610
• Subcellularly, APOBEC3G localizes to the cytoplasm, nucleus, and P-bodies, with its distribution influenced by cellular activation state.
• Tissue expression is observed in spleen, testes, ovary, peripheral blood leukocytes, and CD4+ lymphocytes. In resting CD4 T-cells and monocytes, it exists as a low molecular mass (LMM) form, switching to high molecular mass (HMM) complexes upon activation or differentiation, correlating with increased HIV-1 susceptibility.
• Keywords: Antiviral defense, innate immunity, metal-binding (zinc), phosphoprotein, and ubiquitylation highlight its multifaceted regulation and role in host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen spans the cytidine deaminase domain (aa 60-330), thus the antibody is likely to recognize both native and denatured APOBEC3G.
• For Western blot, expect a band at approximately 46kDa; include appropriate positive controls from human, mouse, or rat lysates.
• IHC-P may require optimization of antigen retrieval conditions; validate staining using tissue known to express APOBEC3G.
• Specificity can be confirmed by comparing signals in APOBEC3G knockout or knockdown samples versus wild-type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated partner for IVD raw material procurement, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. For APOBEC3G-related antiviral and innate immunity research, we provide reliable antibodies to support your assay development and diagnostic workflows.