Anti-APOBEC3C Polyclonal Antibody for WB - Q9NRW3

Core Product Specifications and Parameters

Biological Background: APOBEC3C Function and Localization

• APOBEC3C is a DNA cytidine deaminase that catalyzes the conversion of cytosine to uracil in single-stranded DNA, acting as a restriction factor against retroviruses and retrotransposons.
• Functions via both deamination-dependent and -independent mechanisms to inhibit retroviral replication, inducing G-to-A hypermutations in viral DNA and blocking proviral integration.
• Exhibits antiviral activity against a broad range of viruses including SIV, HBV, HHV-1, and EBV, and may suppress LTR and non-LTR retrotransposon mobility.
• Localizes to both the nucleus and cytoplasm, consistent with its role in genome surveillance and host defense.
• Expressed in immune-related and reproductive tissues such as spleen, peripheral blood lymphocytes, thymus, testes, prostate, and ovary.
• Belongs to the APOBEC3 family of cytidine deaminases, characterized by a zinc-binding deaminase domain essential for catalytic activity.
• Implicated in epigenetic regulation through active DNA demethylation, potentially influencing gene expression.
• Keywords include antiviral defense, innate immunity, host-virus interaction, and metal-binding.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human APOBEC3C sequence (amino acids 1-190), which should generate antibodies recognizing multiple epitopes across the native protein.
• For Western blot (WB), expect a specific band near 23 kDa. Titrate antibody concentration and use appropriate positive control lysates (e.g., spleen, testis, or lymphocyte-derived cell lines).
• In immunofluorescence (IF/ICC), the protein localizes to both the nucleus and cytoplasm; optimize fixation and permeabilization conditions to preserve antigenicity.
• For immunoprecipitation (IP), the polyclonal nature of the antibody may enhance capture efficiency; confirm co-precipitation of binding partners under native conditions.
• In ELISA, validate the antibody pair with a suitable capture/detection system, considering the antibody's recognition of multiple epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD development by providing high-quality raw materials like this anti-APOBEC3C polyclonal antibody, validated for key applications in antiviral research and diagnostics. Our portfolio includes optimized antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents to support your assay development from concept to clinic. Leverage our expertise to source rare target reagents and accelerate your diagnostic pipeline.