Anti-APOBEC3B Polyclonal Antibody for WB, ELISA - Q9UH17

Core Product Specifications and Parameters

| Product Name | APOBEC3B Rabbit pAb | | Remarks/Alias | A3B; ARP4; ARCD3; PHRBNL; APOBEC1L; bK150C2.2; DJ742C19.2; APOBEC3B | | Species | Human | | Gene ID (Human) | 9582 | | Gene ID | 9582 | | Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 151-250 of human APOBEC3B (NP_004891.5) | | Source | Rabbit | | Category | Polyclonal Antibodies | | Application | WB, ELISA | | Cross Reactivity | Human | | SWISS | Q9UH17 | | Protein Weight | 46kDa | | Shipping | Ice bag |

Biological Background: APOBEC3B Function and Localization

• APOBEC3B (DNA dC->dU-editing enzyme APOBEC-3B), also known as phorbolin-1-related protein, is a member of the APOBEC3 family of cytidine deaminases.
• Functions as a DNA deaminase that inhibits retrovirus replication and retrotransposon mobility through both deaminase-dependent and -independent mechanisms.
• Converts cytosine to uracil in minus-sense single-stranded viral DNA, leading to G-to-A hypermutations in proviral DNA; it selectively targets single-stranded DNA and does not deaminate double-stranded DNA or RNA.
• Exhibits antiviral activity against simian immunodeficiency virus (SIV), hepatitis B virus (HBV), and human T-cell leukemia virus type 1 (HTLV-1), and may inhibit the mobility of LTR and non-LTR retrotransposons.
• Localizes predominantly to the nucleus.
• Expressed at high to moderate levels in peripheral blood leukocytes, spleen, testes, heart, thymus, prostate, and ovary; low levels in several other tissues.
• Expression in the urothelium is increased upon infection by BK polyomavirus (BKPyV).
• Keywords include antiviral defense, innate immunity, metal-binding (zinc), RNA-binding, and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a recombinant fusion protein spanning amino acids 151-250 of human APOBEC3B, which lies within the core cytidine deaminase domain; target detection may be optimal in samples where APOBEC3B is highly expressed.
• For Western blot (WB), the predicted molecular weight is approximately 46 kDa. Consider using positive control lysates from tissues with high APOBEC3B expression, such as spleen or testes, to confirm band specificity.
• For ELISA applications, it is advisable to validate the antibody using recombinant APOBEC3B protein or cell lysates overexpressing the target, and to determine the appropriate coating and detection conditions empirically.
• As this is a polyclonal antibody, lot-to-lot consistency should be verified for critical quantitative assays, and appropriate isotype controls should be included in experiments.
• Optimal working dilutions and conditions must be determined experimentally by the end user for each specific assay.

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