Anti-APOBEC3B Monoclonal Antibody for WB - Q9UH17

Core Product Specifications and Parameters

Biological Background: APOBEC3B Function and Localization

• APOBEC3B (Apolipoprotein B mRNA Editing Catalytic Polypeptide-like 3B) is a member of the APOBEC3 family of cytidine deaminases, which play critical roles in innate antiviral immunity by catalyzing cytosine-to-uracil deamination in single-stranded DNA.
• It functions as a potent inhibitor of retrovirus replication, including simian immunodeficiency virus (SIV), hepatitis B virus (HBV), and human T-cell leukemia virus type 1 (HTLV-1), through both deamination-dependent mutations and deamination-independent restriction mechanisms.
• APOBEC3B also suppresses the mobility of endogenous retrotransposons (LTR and non-LTR elements), contributing to genome stability.
• The protein is primarily localized in the nucleus, where it accesses single-stranded DNA substrates during viral reverse transcription or retrotransposon mobilization.
• It is expressed at high to moderate levels in peripheral blood leukocytes, spleen, testes, heart, thymus, prostate, and ovary, with low expression in other tissues; expression is upregulated in urothelium upon BK polyomavirus infection.
• Alternative splicing produces multiple isoforms, and the enzyme requires zinc for catalytic activity; it is also known to bind RNA.
• The full-length protein has a molecular weight of approximately 46 kDa (calculated ~45.9 kDa), consistent with the observed band in Western blotting.

Experimental Guidance and Technical Tips

• The immunogen was designed as a recombinant fusion protein spanning amino acids 120-220 within the N-terminal region of human APOBEC3B, which may confer high specificity in Western blotting and ELISA.
• For Western blotting, expect a band around 46 kDa in human cell or tissue lysates; consider including positive controls such as HeLa or other APOBEC3B-expressing cell lines.
• ELISA assays may be optimized by using the recombinant immunogen as a standard or coating antigen; validate cross-reactivity in the relevant sample system due to limited cross-species data.
• Given the monoclonal nature, this antibody is likely to recognize a single epitope; verify lot-to-lot consistency empirically for quantitative assays.
• Store and handle according to the recommended shipping conditions (ice bag) to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting, supporting projects from concept to clinic. This anti-APOBEC3B monoclonal antibody is just one example of the high-performance reagents we source for antiviral research and infectious disease diagnostics. Beyond individual antibodies, we offer validated antibody pairs, optimized bulk reagents, and customized raw-material procurement to accelerate your assay development.