Anti-APOBEC3A Rabbit Polyclonal Antibody for WB & ELISA - P31941

Core Product Specifications and Parameters

Biological Background: APOBEC3A Function and Localization

• APOBEC3A is a DNA dC->dU-editing enzyme (cytidine deaminase) with restriction activity against viruses, foreign DNA, and retrotransposon mobility.
• It exhibits antiviral activity against adeno-associated virus (AAV) and human T-cell leukemia virus type 1 (HTLV-1), and may inhibit both LTR and non-LTR retrotransposons.
• The enzyme selectively targets single-stranded DNA and can deaminate both methylcytosine and cytosine in foreign DNA.
• APOBEC3A can induce somatic hypermutation in nuclear and mitochondrial DNA, and may play a role in epigenetic regulation via active DNA demethylation.
• Subcellular localization includes both the nucleus and cytoplasm.
• Expression is highest in CD14-positive phagocytic cells, keratinocytes, and peripheral blood monocytes; also found in lung and adipose tissues, with elevated levels in certain cancers and upon BK polyomavirus infection.

Experimental Guidance and Technical Tips

• The immunogen spans nearly the entire human APOBEC3A sequence (aa 1-199), offering broad epitope coverage; confirm detection of the 23 kDa target in your sample system via WB.
• For Western blot, optimize standard conditions; consider using a low-percentage gel to resolve the 23 kDa band.
• In ELISA, the polyclonal format may provide high sensitivity; validate with recombinant protein or cell lysates known to express APOBEC3A.
• Cross-reactivity is documented for human; when testing other species, check sequence homology and validate with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers and research laboratories with a comprehensive portfolio of IVD raw materials, from validated antibody pairs to bulk ancillary reagents. For APOBEC3A-focused projects, we supply optimized polyclonal antibodies like this product, along with custom sourcing for rare targets. Rely on our integrated support to streamline your path from concept to clinic.