Anti-APOBEC2 Rabbit pAb for WB and ELISA - Q9Y235

Core Product Specifications and Parameters

Biological Background: APOBEC2 Function and Localization

• APOBEC2 (Apolipoprotein B mRNA Editing Enzyme Catalytic Subunit 2) is also known as ARP1, ARCD1, and mRNA(cytosine(6666)) deaminase 2.
• It belongs to the APOBEC family of cytidine deaminases, sharing structural and functional similarities with other family members.
• APOBEC2 is a probable C-to-U RNA editing enzyme, though its physiological substrate remains unknown. It does not exhibit detectable apoB mRNA editing activity.
• The enzyme possesses low intrinsic cytidine deaminase activity in vitro and may contribute to epigenetic regulation via active DNA demethylation.
• APOBEC2 is expressed exclusively in heart and skeletal muscle tissues, suggesting a tissue-specific function.
• Structural studies reveal a conserved zinc-dependent deaminase domain, classifying it as a hydrolase and metal-binding protein involved in mRNA processing.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering the full-length human APOBEC2 (amino acids 1–224). This design may provide broad epitope coverage for detecting native and denatured protein.
• For Western blot (WB) applications, a band at approximately 26 kDa is expected. Consider using heart or skeletal muscle lysates as positive controls based on tissue specificity.
• For ELISA, the polyclonal nature of this antibody may enhance sensitivity; however, assay optimization is recommended for quantitative applications.
• Cross-reactivity has been reported in human, mouse, and rat; validate in relevant species and sample types before experimental use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes reliable access to a comprehensive range of IVD raw materials and technical services. This anti-APOBEC2 polyclonal antibody supports research in epigenetic regulation and muscle biology. Our team provides validated antibody pairs, custom bulk production, and expertise in rare target sourcing—ensuring a streamlined path from discovery to diagnostic application.