Anti-APOB Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P04114

Core Product Specifications and Parameters

Biological Background: ApoB Function and Localization

• Apolipoprotein B (ApoB) exists as two main isoforms: ApoB-48, synthesized in the intestine and incorporated into chylomicrons, and ApoB-100, produced in the liver and found in VLDL and LDL particles.
• ApoB mRNA undergoes tissue-specific RNA editing, which introduces a premature stop codon to generate the truncated ApoB-48 isoform in the intestine.
• ApoB-100 functions as a ligand for the LDL receptor (apoB/E receptor), mediating the cellular binding and internalization of LDL particles.
• ApoB is a central player in lipid metabolism and transport, involved in cholesterol homeostasis and lipoprotein assembly.
• Subcellularly, ApoB is located in the cytoplasm, secreted into the plasma, and associates with lipid droplets.
• The protein undergoes multiple post-translational modifications, including phosphorylation, acetylation, glycosylation, disulfide bond formation, and palmitoylation.
• Mutations in APOB are associated with familial hypercholesterolemia, atherosclerosis, and other lipid disorders.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1890–2190 of human ApoB-100 (NP_000375.3); this region is absent in the shorter ApoB-48 isoform, making the antibody specific for ApoB-100 unless otherwise indicated.
• Due to its large molecular weight (~516 kDa), use low‑percentage (e.g., 6–8%) SDS‑PAGE gels, extended transfer times, and a high‑molecular‑weight marker for Western blot.
• Consider validating the antibody in the relevant sample system (e.g., liver lysates for ApoB-100, intestinal lysates for ApoB‑48) to confirm isoform detection.
• For IHC‑P and IF/ICC, optimize antigen retrieval protocols (e.g., heat‑induced epitope retrieval) and fixative conditions for your specific tissue types.
• In ELISA applications, the antibody may be used as capture or detection reagent; empirical optimization of coating buffer, blocking, and detection conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw‑material procurement for diagnostic manufacturers and laboratories worldwide. This anti‑APOB polyclonal antibody is part of our comprehensive portfolio for lipid metabolism and cardiovascular disease research, supported by validated antibody pairs, optimized bulk reagents, and custom sourcing for rare or emerging targets. From early R&D to clinical application, we deliver integrated supply solutions to accelerate your assay development pipeline.