Anti-APOA5 Polyclonal Antibody for WB, ELISA - Q6Q788

Core Product Specifications and Parameters

Biological Background: APOA5 Function and Localization

• APOA5 is a minor apolipoprotein mainly associated with HDL, VLDL, and chylomicrons, and is a critical regulator of plasma triglyceride levels.
• It stimulates lipoprotein lipase (LPL) activity through apo-CII and inhibits hepatic VLDL-triglyceride production, thereby modulating lipid metabolism.
• APOA5 binds heparin, which may facilitate its interactions with lipoproteins and cell surfaces.

Related references: PMID:17326667

• It poorly activates LCAT and does not promote cholesterol efflux from macrophages, indicating a specialized role distinct from other apolipoproteins.
• The protein is secreted and localizes to early and late endosomes, as well as the trans-Golgi network.
• Expression is primarily restricted to the liver and plasma.
• Post-translational modifications include phosphorylation, and variants in APOA5 are associated with diseases affecting lipid homeostasis.
• As a member of the apolipoprotein family, APOA5 contains coiled-coil domains and is involved in lipid transport and VLDL assembly.

Experimental Guidance and Technical Tips

• For Western blotting, liver or plasma samples from human or mouse are suitable starting materials; optimization of antibody dilution and denaturing/reducing conditions is recommended.
• The immunogen corresponds to amino acids 207–366 of human APOA5, which may influence epitope recognition; consider using the recombinant protein as a positive control.
• In ELISA applications, direct or sandwich formats can be explored; pairing with a capture antibody may require empirical testing.
• Cross-reactivity with mouse APOA5 is reported, but sequence alignment in the target species should be verified for other applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering a wide range of validated antibodies, recombinant proteins, and ancillary reagents. For researchers and manufacturers focused on lipid metabolism and cardiovascular disease, we provide access to high-quality APOA5 antibodies suitable for diagnostic and research applications. Our portfolio includes polyclonal and monoclonal antibodies, matched pairs, and custom bulk supply services, streamlining your transition from concept to clinic.