Anti-APOA1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P02647

Core Product Specifications and Parameters

Biological Background: Apolipoprotein A-I Function and Localization

• Apolipoprotein A-I (ApoA-I) is the major protein component of plasma high-density lipoprotein (HDL) and is also found in chylomicrons, playing a central role in cholesterol homeostasis.
• It mediates reverse cholesterol transport by promoting cholesterol efflux from peripheral tissues and acting as an essential cofactor for lecithin-cholesterol acyltransferase (LCAT), which esterifies cholesterol for packaging into HDL particles.
• ApoA-I is a secreted protein synthesized primarily in the liver and small intestine, then released into circulation where it associates with lipid particles.
• The gene APOA1 belongs to the apolipoprotein A1/C3/A4/A5 gene cluster on chromosome 11 and encodes a preproprotein that undergoes proteolytic processing to yield the mature 243-amino acid protein.
• Post-translational modifications include oxidation at Met-110 and Met-136, which are elevated in individuals with coronary artery disease risk factors such as smoking and eNOS gene variants; the protein can also be glycated, nitrated, and palmitoylated.
• ApoA-I is implicated in amyloidosis, where certain variants form amyloid fibrils, and in atherosclerosis, where oxidized forms are enriched in aortic lesions and correlate with disease severity.
• As part of the SPAP complex, ApoA-I activates spermatozoa motility, indicating an extrahepatic function beyond lipid transport.
• The protein’s importance in lipid metabolism is underscored by its association with HDL levels and cardiovascular disease, making it a key biomarker and therapeutic target.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 25–267, which corresponds to the mature secreted form of human ApoA-I; consider verifying reactivity against endogenous protein in relevant biological samples (e.g., serum, plasma, or liver lysates).
• For Western blot, the predicted molecular weight is ~28 kDa; a band near this size in human, mouse, and rat samples confirms cross-reactivity. Optimize sample preparation to reduce lipid interference if using plasma or serum.
• In immunohistochemistry (IHC-P), test the antibody on paraffin-embedded liver or atherosclerotic arterial tissue sections, using appropriate antigen retrieval, as ApoA-I is extracellular and may require mild retrieval conditions.
• For ELISA, validate the antibody pair in a sandwich format if used as capture or detection reagent; the polyclonal nature may provide signal amplification but requires careful titration.
• When working with oxidized forms of ApoA-I, note that methionine oxidation can alter epitope recognition; reducing agents and fresh samples are recommended.

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CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibodies, antigens, and ancillary reagents for lipid metabolism and cardiovascular disease research. Our global sourcing network and technical expertise enable reliable supply of polyclonal and monoclonal antibodies, optimized antibody pairs, and bulk ancillary materials to accelerate assay development from concept to clinic. Whether you require standard catalog products or customized rare-target sourcing, CamelBio is your trusted partner in IVD raw-material procurement.