Anti-APLP1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P51693

Core Product Specifications and Parameters

Biological Background: APLP1 Function and Localization

• APLP1 (Amyloid beta precursor like protein 1), also known as Amyloid-like protein 1, is a transmembrane glycoprotein belonging to the amyloid precursor protein family.
• It is expressed in the cerebral cortex, specifically localizing to the postsynaptic density (PSD), and may play a role in postsynaptic function.
• APLP1 couples to JIP signal transduction pathways via C-terminal binding and interacts with cellular G protein signaling.
• The C-terminal gamma-secretase processed fragment ALID1 activates transcription through APBB1 (Fe65) binding, while the gamma-CTF peptide C30 enhances neuronal apoptosis.
• The protein exhibits heparin-binding and cell adhesion properties, and may regulate neurite outgrowth through interactions with extracellular matrix components like collagen I.
• APLP1 contains metal-binding sites for copper and zinc, and its functions link to neurodegeneration and apoptosis.

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant protein spanning the mid-region (aa 285-490) of human APLP1, so it may recognize full-length and some processed isoforms.
• For Western blot, consider using brain tissue lysates or neuronal cell lines; the predicted molecular weight is ~72 kDa, but post-translational modifications may cause band shifts.
• In IHC-P, positive signal is expected in the cerebral cortex; validate with appropriate tissue controls.
• For IF/ICC, permeabilization may be necessary to detect cytoplasmic and membrane-associated APLP1; consider co-staining with synaptic markers.
• ELISA applications may require optimization of coating and detection conditions; the antibody can be used as a capture or detection reagent.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutions with integrated access to IVD raw materials, technical services, and consulting. For neuroscientific and apoptosis-related research, we offer validated antibody pairs, optimized polyclonal antibodies like this anti-APLP1 reagent, bulk ancillary reagents, and rare-target raw-material sourcing. Partner with CamelBio as your reliable B2B sourcing bridge from concept to clinic.