Anti-APLP1 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P51693

Core Product Specifications and Parameters

Biological Background: APLP1 Function and Localization

• Amyloid beta precursor like protein 1 (APLP1), also known as amyloid-like protein 1, is a member of the amyloid precursor protein family and a type I transmembrane glycoprotein.
• APLP1 plays a role in postsynaptic function, with its C-terminal gamma-secretase fragment ALID1 activating transcription through APBB1 (Fe65) binding, and coupling to JIP signal transduction.
• The protein can regulate neurite outgrowth by binding to extracellular matrix components such as heparin and collagen I; it may also interact with cellular G-protein signaling pathways.
• A gamma-secretase processed peptide, C30, acts as a potent enhancer of neuronal apoptosis.
• APLP1 localizes to the cell membrane and cytoplasm, and is expressed in the cerebral cortex, specifically concentrated at the postsynaptic density (PSD).
• Key features include alternative splicing, copper and zinc binding, disulfide bonds, glycosylation, endocytosis, and heparin binding; it is associated with neurodegeneration.

Experimental Guidance and Technical Tips

• The immunogen sequence (amino acids 470-580) lies in the extracellular region of APLP1; the rabbit polyclonal antibody is suitable for detecting endogenous protein across human, mouse, and rat samples as listed.
• For Western blot (WB), expect a band at approximately 72 kDa. Optimize the antibody dilution for your specific lysis buffer and sample type.
• In immunofluorescence (IF-P), permeabilization is required to detect cytoplasmic and membrane-associated pools. Validate fixation and permeabilization conditions for your cell line or tissue.
• For ELISA, the polyclonal antibody can serve as a capture or detection reagent. Titrate to determine the optimal working concentration and consider pairing with a monoclonal antibody for enhanced specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material sourcing, providing high-quality antibodies like this anti-APLP1 polyclonal antibody along with validated antibody pairs, optimized monoclonal and polyclonal reagents, and bulk ancillary materials. We support neurodegenerative disease research and diagnostic development, helping you secure rare-target raw materials efficiently from concept to clinic.