Anti-APLF Rabbit Polyclonal Antibody for WB, ELISA - Q8IW19

Core Product Specifications and Parameters

Biological Background: APLF Function and Localization

• APLF acts as a histone chaperone essential for single-strand and double-strand DNA break repair.
  Related references: PMID:17353262, PMID:17396150, PMID:21211721
• It is recruited to DNA damage sites by binding branched poly-ADP-ribose chains, where it promotes histone eviction and recruitment of the histone variant MACROH2A1.
  Related references: PMID:21211722, PMID:29905837, PMID:30104678
• APLF possesses apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities and can introduce nicks at hydroxyuracil and other pyrimidine base damage in vitro.
  Related references: PMID:17353262, PMID:17396150
• In the non-homologous end joining (NHEJ) pathway, APLF collaborates with PARP3 to retain the LIG4-XRCC4 complex on chromatin, accelerating DNA ligation.
  Related references: PMID:21211721, PMID:23689425
• APLF negatively regulates cell pluripotency by promoting histone exchange and is required for embryo implantation during the epithelial-to-mesenchymal transition in females.
• Subcellularly, APLF localizes to the nucleus, chromosomes, and cytoplasm/cytosol, consistent with its DNA repair and chromatin regulatory functions.
• Structural motifs include an FHA domain, zinc-finger repeats, and sites for phosphorylation and ADP-ribosylation, highlighting its role in signal-dependent DNA damage responses.

Experimental Guidance and Technical Tips

• For Western blot, expect a band near 57 kDa in human-derived samples; include a positive control such as the recombinant immunogen (aa 200-380) to confirm detection.
• As cross-reactivity with rat has been observed, validate the antibody in rat cell or tissue lysates if your research involves rodent models.
• For ELISA applications, coating with the recombinant APLF fragment may improve signal specificity; assay conditions should be optimized empirically.
• Given APLF's nuclear and cytoplasmic localization, consider subcellular fractionation to enrich the target for more sensitive detection in WB.
• Since APLF is involved in DNA damage, treatment with DNA-damaging agents may increase protein recruitment to chromatin; time-course experiments may reveal dynamic localization changes.

CamelBio: Your One-Stop Sourcing Bridge

This APLF polyclonal antibody is part of CamelBio’s comprehensive portfolio for DNA repair and genomic stability research. As a dedicated IVD raw-material partner, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, ancillary reagents, and customized sourcing for rare targets—streamlining your path from discovery to clinical application.