Anti-APIP Polyclonal Antibody for WB, IHC-P, ELISA - Q96GX9

Core Product Specifications and Parameters

Biological Background: Methylthioribulose-1-phosphate Dehydratase (APIP) Function and Localization

• APIP, also known as APAF1-interacting protein (hAPIP), is a methylthioribulose-1-phosphate dehydratase enzyme encoded by the APIP gene in humans.
• It catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P), a key step in the methionine salvage pathway.
• The methionine salvage pathway is crucial for cancer cell metabolism, apoptosis regulation, microbial proliferation, and inflammation.
• APIP inhibits CASP1-related inflammatory response (pyroptosis), the CASP9-dependent apoptotic pathway, and cytochrome c-dependent, APAF1-mediated cell death.
• APIP is primarily localized in the cytoplasm.
• Two isoforms exist: Isoform 1 is ubiquitously expressed, while isoform 2 shows lower expression and is detected in heart, brain, pancreas, liver, placenta, skeletal muscle, and kidney.
• According to UniProt, APIP is annotated with keywords including Alternative splicing, Apoptosis, Lyase, Metal-binding, and Zinc, highlighting its structural and functional diversity.

Experimental Guidance and Technical Tips

• As a polyclonal antibody, this product may recognize multiple isoforms or splice variants of APIP; consider using isoform-specific controls for precise identification.
• For Western blot (WB), the predicted molecular weight of APIP is approximately 27 kDa; validate band size in your experimental system.
• In immunohistochemistry (IHC-P), optimize antigen retrieval conditions and validate the staining pattern in positive and negative tissue controls.
• For ELISA applications, the recombinant immunogen (amino acids 1-242) can be used as a positive control to establish assay parameters.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with a comprehensive, one-stop solution for IVD raw material sourcing. For research into apoptosis, methionine metabolism, or inflammatory pathways, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and support in sourcing rare-target raw materials. Let CamelBio accelerate your project from concept to clinic with tailored procurement and supply solutions.