Anti-APEX1/APE1 Rabbit Polyclonal Antibody (KO Validated) for WB, IF/ICC, IP, ELISA - P27695

Core Product Specifications and Parameters

Biological Background: APEX1/APE1 Function and Localization

• APEX1, also known as APE1, REF1, HAP1, APEX nuclease (APEN), is a multifunctional protein with two major activities: DNA repair and redox regulation of transcriptional factors, playing a central role in the cellular response to oxidative stress. Related references: PMID:11118054 PMID:11452037 PMID:15831793
• As an apurinic/apyrimidinic (AP) endodeoxyribonuclease, APEX1 initiates the base excision repair (BER) pathway by catalyzing hydrolytic incision of the DNA backbone at AP sites, and also possesses 3'-5' exodeoxyribonuclease and DNA 3' phosphodiesterase activities to process blocked DNA ends. Related references: PMID:15380100 PMID:16617147 PMID:18439621 PMID:11832948 PMID:1719477 PMID:15831793 PMID:7516064
• Through its redox function, APEX1 regulates the DNA binding and transcriptional activity of transcription factors, including the FOS/JUN AP-1 complex, by controlling the redox status of their DNA-binding domains. Related references: PMID:10023679 PMID:11118054 PMID:11452037
• APEX1 localizes to multiple subcellular compartments, including the nucleus (nucleolus and nuclear speckles), cytoplasm, mitochondria, and endoplasmic reticulum, reflecting its diverse functions.
• APEX1 participates in immunoglobulin isotype class switch recombination by processing AP sites generated by AICDA and UNG, creating staggered nicks that resolve into double-strand breaks via non-homologous end joining.
• It also functions as an endoribonuclease, controlling single-stranded RNA metabolism by mediating MYC mRNA turnover and participating in rRNA quality control during cell cycle progression. Related references: PMID:19188445 PMID:19401441 PMID:21762700
• Post-translational modifications including acetylation, phosphorylation, S-nitrosylation, and ubiquitination modulate APEX1's enzymatic and signaling activities.

Experimental Guidance and Technical Tips

• For Western blot, a band at approximately 36 kDa is expected; KO validation data supports target specificity, but users should confirm performance in their own experimental system.
• In immunofluorescence/ICC, APEX1 exhibits predominant nuclear localization with possible cytoplasmic/mitochondrial staining; optimize fixation and permeabilization protocols to preserve epitope accessibility.
• The antibody has been reported for use in immunoprecipitation and ELISA; individual optimization of antibody dilution and incubation conditions is recommended for these applications.
• Given the polyclonal nature and immunization with full-length recombinant protein (aa 1-318), the antibody likely contains multiple epitope specificities, potentially providing robust detection across various assay formats and post-translational modifications.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop sourcing partner, CamelBio offers a comprehensive portfolio of IVD raw materials including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers studying APEX1/APE1 in DNA repair and oxidative stress, CamelBio can supply this rabbit polyclonal antibody alongside complementary reagents and technical support. Our integrated supply chain streamlines procurement for diagnostic development, from early-stage research to clinical application.