Anti-APEX1/APE1 Polyclonal Antibody for WB, IF/ICC, ELISA - P27695

Core Product Specifications and Parameters

Biological Background: APEX1/APE1 Function and Localization

• APEX1 is a multifunctional protein with two major activities: DNA repair via apurinic/apyrimidinic (AP) endonuclease function in base excision repair (BER), and redox regulation of transcription factors such as FOS/JUN (AP-1 complex). Related references: PMID:11118054 PMID:11452037 PMID:15831793
• The AP endonuclease activity catalyzes hydrolytic incision of phosphodiester backbone at AP sites in DNA, DNA/RNA hybrids, and single-stranded RNA, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Related references: PMID:15380100 PMID:16617147 PMID:18439621
• It possesses 3'-5' exodeoxyribonuclease activity on mismatched nucleotides and DNA 3' phosphodiesterase activity, removing oxidative lesions like phosphoglycolate and 8-oxoguanine. Related references: PMID:11832948 PMID:1719477 PMID:7516064
• APEX1 participates in immunoglobulin class switch recombination by processing AP sites at switch regions, leading to DNA double-strand breaks resolved by non-homologous end joining.
• It acts as an endoribonuclease involved in RNA quality control and mRNA turnover, including regulation of MYC mRNA stability. Related references: PMID:19188445 PMID:19401441 PMID:21762700
• The protein localizes to multiple subcellular compartments: nucleus (including nucleolus and speckles), cytoplasm, mitochondria, and endoplasmic reticulum, reflecting its diverse functions.
• Key post-translational modifications include acetylation, phosphorylation, S-nitrosylation, and ubiquitination; acetylation at Lys-6/7 stimulates MDR1 promoter activity, contributing to drug resistance. Related references: PMID:18809583
• APEX1 plays a role in DNA demethylation, contributing to epigenetic regulation of gene expression. Related references: PMID:21496894

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning amino acids 1-318 of human APEX1 (full-length), suggesting the antibody may recognize both native and denatured forms. For WB, a band near 36 kDa is expected; use appropriate positive controls (e.g., HeLa or HEK293 cell lysates) and validate with siRNA knockdown if necessary.
• For IF/ICC, anticipate nuclear and possibly cytoplasmic or mitochondrial staining. Co-staining with organelle markers (e.g., lamin for nucleus, MitoTracker for mitochondria) may help confirm specificity.
• In ELISA applications, consider coating with recombinant APEX1 protein as a standard and testing across a range of concentrations to optimize sensitivity.
• Given APEX1's role in BER, the antibody may be useful for detecting changes in expression under oxidative stress; include relevant controls (e.g., H₂O₂-treated cells) to correlate with DNA damage response pathways.
• Cross-reactivity is validated for human; for other species, check sequence homology and perform pilot experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, offering comprehensive support from concept to clinic. This anti-APEX1/APE1 polyclonal antibody supports advanced research in DNA repair, oxidative stress, and oncology. Beyond antibodies, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your diagnostic development.