Anti-APEH Polyclonal Antibody for WB, IP, ELISA - P13798

Core Product Specifications and Parameters

Biological Background: APEH Function and Localization

• Official full name: Acylamino-acid-releasing enzyme, also known as acyl-peptide hydrolase (APH), acylaminoacyl-peptidase, and oxidized protein hydrolase (OPH).
• Functions as an aminopeptidase with exopeptidase activity, specifically hydrolyzing the N-terminal peptide bond of N-acetylated peptides to generate an N-acetylated amino acid and a peptide with a free N-terminus; preferentially cleaves Ac-Ala, Ac-Met, and Ac-Ser. Related references: PMID:10719179 PMID:1740429 PMID:2006156
• Exhibits endopeptidase activity involved in the degradation of oxidized and glycated proteins, contributing to cellular quality control of damaged proteins. Related references: PMID:10719179
• May act sequentially with aminoacylase-1 (ACY1) in the degradation of N-acylated peptides: APEH first removes N-acylamino acids from N-acylated peptides, then ACY1 further hydrolyzes the N-acylamino acid into free amino acid and a carboxylate.
• Subcellular localization is predominantly cytoplasmic.
• Expressed in erythrocytes at the protein level, suggesting a role in red blood cell function.
• Post-translational modifications include acetylation and phosphorylation, as identified by proteomics.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-260 of human APEH; for Western blot, the antibody is expected to detect a band at approximately 81 kDa. Use appropriate reducing conditions and loading controls.
• For immunoprecipitation, validate the antibody’s performance with your target cell/tissue lysate and consider optimizing antibody-to-lysate ratios.
• In ELISA, the antibody may recognize both native and denatured APEH; empirical determination of coating conditions and concentrations is recommended.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw-material partner, CamelBio supports diagnostic manufacturers and researchers with validated antibody products, including polyclonal antibodies like this anti-APEH, and can assist in sourcing ancillary reagents and rare-target materials. With expertise spanning from concept to clinic, CamelBio offers one-stop access to the raw materials and technical support needed to accelerate your APEH-related protein degradation and quality control research.