Anti-APEH Polyclonal Antibody for WB, IF/ICC, ELISA - P13798

Core Product Specifications and Parameters

Biological Background: APEH Function and Localization

• APEH (Acylamino-acid-releasing enzyme), also known as Acyl-peptide hydrolase (APH), Acylaminoacyl-peptidase, and Oxidized protein hydrolase (OPH), is encoded by the APEH gene (alias D3F15S2, D3S48E, DNF15S2) in humans.
• APEH catalyzes the removal of N-acetylated amino acids from peptides, generating a free N-terminus, as demonstrated by early biochemical characterization. Related references: PMID:1740429 PMID:2006156
• It also exhibits endopeptidase activity, contributing to the degradation of oxidized and glycated proteins, suggesting a role in cellular protein quality control. Related references: PMID:10719179
• The enzyme preferentially cleaves Ac-Ala, Ac-Met, and Ac-Ser residues and may act sequentially with ACY1 in the complete degradation of N-acylated peptides.
• APEH is localized to the cytoplasm, consistent with its role in intracellular peptide and protein metabolism.
• At the protein level, expression is detected in erythrocytes, indicating a specific tissue distribution.
• Post-translational modifications include acetylation and phosphorylation, as noted in keywords and proteomics studies; the protein has a calculated molecular weight of 81,225 Da.

Experimental Guidance and Technical Tips

• For Western blot (WB), a band corresponding to APEH can be expected around 81-82 kDa based on the predicted molecular weight; verify using appropriate positive control lysates from human, mouse, or rat tissues/cells.
• Since the immunogen spans amino acids 1-260 (N-terminal region), the antibody may recognize full-length APEH; consider validating specificity with knockdown or knockout samples.
• In immunofluorescence/immunocytochemistry (IF/ICC), anticipate cytoplasmic staining; optimize fixation and permeabilization conditions for the target cell type.
• For ELISA, the antibody can potentially serve as a capture or detection reagent; pairing with a matched antibody clone may enhance sensitivity.
• The reported cross-reactivity with mouse and rat extends its utility to common model organisms; however, always confirm reactivity in the specific experimental system.

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