Anti-APC Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P25054

Core Product Specifications and Parameters

Biological Background: APC Function and Localization

• APC (Adenomatous polyposis coli protein) is a 312 kDa tumor suppressor that functions as a negative regulator of the Wnt signaling pathway by promoting rapid degradation of CTNNB1 (β-catenin).
• It plays a role in cell migration, being activated by hepatocyte growth factor (HGF) and required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells.
• APC associates with both microtubules and actin filaments, mediating the organization of F-actin into ordered bundles and stabilizing microtubules, particularly at the cell cortex, through interactions with ERBB2, Rho GTPases, and DIAPH1. Related references: PMID:17293347
• The protein localizes to adherens junctions, cytoplasm, cytoskeleton, lamellipodia, ruffle membranes, and the cell membrane, reflecting its dynamic roles in cell adhesion and motility.
• Multiple alternative isoforms exist; isoform 1A is very strongly expressed in the brain, while isoform 1B is the predominant form in most other tissues, including the gastrointestinal tract and blood.
• APC contains several functional domains and motifs, including coiled-coil regions, armadillo repeats, and β-catenin binding sites, and is subject to phosphorylation, acetylation, and ubiquitination, which modulate its tumor suppressor activities.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning amino acids 2600–2760 of human APC, located in the C‑terminal region. This design may contribute to recognition of multiple isoforms and species.
• For western blotting, the predicted molecular weight is approximately 312 kDa; consider using a low‑percentage (e.g., 6–8%) SDS‑PAGE gel and extended transfer times for optimal resolution.
• In immunohistochemistry (IHC‑P) and immunofluorescence (IF/ICC), signal specificity should be confirmed using appropriate positive and negative control tissues or cells that express APC.
• ELISA can be employed for detection; optimal antibody dilution and blocking conditions must be determined empirically for each assay format.
• Cross‑reactivity with human, mouse, and rat APC is reported; when working with other species, validate reactivity in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw‑material sourcing, including validated antibodies like this anti‑APC polyclonal antibody, to support research and diagnostic development in oncology, Wnt signaling, and cell migration. Our global supply network ensures access to optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized solutions for rare targets. Partner with CamelBio to streamline your project from concept to clinic.