Anti-APBB1 Rabbit Polyclonal Antibody for WB, ELISA - O00213

Core Product Specifications and Parameters

Biological Background: APBB1 Function and Localization

• APBB1 is a transcription coregulator with both coactivator and corepressor activities, and forms a transcriptionally active complex with the APP intracellular domain. Related references: PMID:15031292, PMID:18468999, PMID:18922798

• It plays a central role in DNA damage response by recognizing and binding phosphorylated H2AX at Tyr-142 (H2AXY142ph) at double-strand breaks, recruiting pro-apoptotic factors like JNK1, and is required for histone H4 acetylation at DSBs. Related references: PMID:19234442

• APBB1 specifically binds modified histones and chromatin-modifying enzymes like KAT5/TIP60 to activate transcription, and acts as a transcriptional repressor in complex with TSHZ3, SET, and HDAC to inhibit CASP4 expression. Related references: PMID:33938178, PMID:19343227

• It localizes to multiple cellular compartments including the cell membrane, cytoplasm, nucleus, growth cones, and nuclear speckles, enabling its diverse signaling and regulatory functions.

• Highly expressed in the brain, APBB1 levels are reduced in post-mortem Alzheimer disease patients, suggesting a link to neuronal health.

• It mediates insulin-induced neurite outgrowth by activating the RAC1-ARF6 axis, and also contributes to hippocampal neurite branching, neuromuscular junction formation, lens transparency, and muscle cell strength. Related references: PMID:36250347

• Post-translational modifications such as phosphorylation, acetylation, and ubiquitin-like conjugation regulate APBB1 activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 359-708 of human APBB1 (NP_663722.1). For Western blot, consider using a relevant positive control lysate (e.g., human or mouse brain tissue) and validate the observed band against the predicted molecular weight of 77 kDa.
• For ELISA applications, immobilization of the recombinant immunogen or cell lysates may be employed; optimization of coating conditions and antibody dilution is recommended.
• Given cross-reactivity with mouse, this antibody may be suitable for detecting APBB1 in mouse-derived samples; however, species-specific validation in the intended model system is advised.
• As APBB1 is involved in DNA damage response, treatments with genotoxic agents (e.g., etoposide) could be explored to study induced nuclear translocation or post-translational modifications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material procurement, offering a comprehensive portfolio that includes validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. This anti-APBB1 polyclonal antibody, targeting a crucial adapter in Alzheimer's disease and DNA damage signaling, exemplifies our commitment to supplying high-quality reagents for translational research. For bulk orders or custom sourcing of rare-target antibodies, our team provides tailored support to streamline your assay development from concept to clinic.