Anti-AP3S2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P59780

Core Product Specifications and Parameters

Biological Background: AP3S2 Function and Localization

• AP3S2 encodes the sigma-2 subunit (also known as sigma3B-adaptin) of the adaptor-related protein complex 3 (AP-3), a heterotetrameric complex involved in intracellular trafficking.
• The AP-3 complex is not clathrin-associated and localizes to the Golgi apparatus and peripheral cytoplasmic vesicle membranes, where it facilitates the budding of vesicles from the Golgi and directs cargo to lysosomes.
• In concert with the BLOC-1 complex, AP-3 is essential for sorting cargo into vesicles at the cell body for delivery into neurites and nerve terminals, playing a critical role in neuronal function.
• AP3S2 is ubiquitously expressed in all adult tissues examined, reflecting its fundamental role in cellular membrane trafficking.
• Subcellular localization: Golgi apparatus membrane and cytoplasmic vesicle membrane, consistent with its function in early secretory and endosomal pathways.
• Alternative splicing of AP3S2 generates multiple isoforms, which may contribute to functional diversity in different cell types or conditions.
• Keywords: Alternative splicing; Cytoplasmic vesicle; Golgi apparatus; Membrane; Protein transport; Proteomics identification; Reference proteome; Transport.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1–193) of human AP3S2, and the resulting polyclonal antibody is expected to recognize multiple epitopes, including potential isoforms.
• For western blot (WB), the predicted molecular weight of full-length AP3S2 is approximately 22 kDa; however, the actual band may vary depending on the isoform and post-translational modifications. Validate in your own sample system.
• For immunofluorescence (IF/ICC), proper fixation and permeabilization are required to detect the intracellular Golgi and vesicular localization. Consider using appropriate organelle markers for co-localization.
• Cross-reactivity is reported for human, mouse, and rat; for other species, sequence homology should be checked and empirical validation is recommended.
• The antibody is validated for ELISA applications, making it suitable for quantitative detection or capture assays. Optimize coating and detection conditions for your specific use case.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your streamlined sourcing partner for IVD raw materials, providing validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to support diagnostic and research workflows. Our portfolio includes AP3S2 and AP-3 complex-related products, enabling studies on lysosomal trafficking, neuronal cargo sorting, and related disorders. Whether you need routine supply or rare target raw-material sourcing, we offer a seamless procurement experience from concept to clinic.