Anti-AP3D1 Rabbit Polyclonal Antibody for WB, ELISA - O14617

Core Product Specifications and Parameters

Biological Background: AP3D1 Function and Localization

• AP3D1 encodes the delta-1 subunit of the heterotetrameric AP-3 adaptor complex, which is not associated with clathrin.
• The AP-3 complex facilitates vesicle budding from the Golgi membrane and mediates trafficking to lysosomes.
• AP3D1 is essential for CD8+ T-cell and NK cell degranulation (PubMed:26744459). Related references: PMID:26744459
• In concert with the BLOC-1 complex, AP-3 targets cargos into vesicles for delivery into neurites and nerve terminals.
• Subcellularly, AP3D1 localizes to the cytoplasm and Golgi apparatus membrane.
• It is expressed in all adult tissues, with highest levels in skeletal muscle, heart, pancreas, and testis.
• Mutations in AP3D1 are associated with Hermansky-Pudlak syndrome (HPS), a disorder of lysosome-related organelle biogenesis.
• Post-translational modifications include acetylation and phosphorylation; the protein features coiled-coil and repeat domains.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 520–700 of human AP3D1 (NP_003929.4). Consider using this fragment or full-length protein as a positive control in your assays.
• For Western blotting, a reducing SDS-PAGE system is typically suitable; validate transfer and blocking conditions with the relevant sample system.
• In ELISA, pairing with a matched detection antibody may improve sensitivity; titrate the antibody against recombinant antigen or lysates.
• Given cross-reactivity with mouse, this antibody may be used for studies in mouse models; validate performance in the desired tissue or cell type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibodies for targets like AP3D1 involved in lysosomal trafficking and genetic disorders. Our offerings encompass optimized polyclonal antibodies, ancillary reagents, and rare-target sourcing—streamlining your transition from concept to clinic. Let CamelBio bridge your raw material needs with reliable, one-stop access.