Anti-AP2S1 Rabbit Monoclonal Antibody for WB and ELISA - P53680

Core Product Specifications and Parameters

Biological Background: AP2S1 Function and Localization

• AP2S1 encodes the sigma subunit of the adaptor protein complex 2 (AP-2), a heterotetrameric complex essential for clathrin-mediated endocytosis at the plasma membrane. It is also known as AP17, CLAPS2, sigma2-adaptin, and clathrin coat-associated protein AP17.
• As a core component of AP-2, the sigma subunit contributes to the selective recruitment of transmembrane cargo into clathrin-coated vesicles (CCVs) by recognizing Y-X-X-Φ and [ED]-X-X-X-L-[LI] endocytosis signals within the cytosolic tails of cargo proteins.
• AP-2 functions as a major clathrin adaptor, bridging the clathrin lattice and membrane components to drive vesicle formation and cargo sorting. It is also implicated in recycling synaptic vesicle membranes from the presynaptic surface.
• Among the four subunits (α, β2, μ2, σ2), the sigma subunit plays a role in stabilizing the complex and participates in the recognition of dileucine-based sorting motifs.
• Subcellularly, AP2S1 localizes to the cell membrane and coated pits, consistent with its role in early endocytic events.
• Post-translational modifications include phosphorylation (as indicated by UniProt keyword), and alternative splicing produces different isoforms.
• AP2S1 may additionally contribute to extracellular calcium homeostasis, although this function is less characterized.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody was a synthetic peptide corresponding to the N-terminal region (amino acids 1-100) of human AP2S1. This region is likely to be surface-exposed and may provide good epitope accessibility. Consider performing peptide blocking experiments to confirm specificity if necessary.
• For Western blot (WB), the predicted molecular weight is approximately 17 kDa. Ensure appropriate gel percentage (e.g., 12-15%) and transfer conditions for low-molecular-weight proteins. Validate the antibody in your specific sample matrix, as post-translational modifications or isoform variants could influence band patterns.
• The antibody cross-reacts with mouse, making it suitable for studies in mouse models or cell lines. For ELISA, consider using a direct or indirect format with appropriate antigen coating. Titration and optimization are recommended for each assay.
• Given the role of AP-2 in endocytosis, this antibody may be useful for investigating cargo trafficking, receptor internalization, and synaptic vesicle dynamics. Combine with organelle markers or functional assays to contextualize localization and activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to a wide range of IVD raw materials. For projects focused on endocytosis, membrane trafficking, or adaptor protein complexes, we can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials. Our sourcing and technical support help bridge the gap from concept to clinic, enabling efficient development of diagnostic assays and research tools.