Anti-AP2M1 Rabbit Polyclonal Antibody for WB and ELISA - Q96CW1

Core Product Specifications and Parameters

Biological Background: AP2M1 Function and Localization

• AP2M1 encodes the mu subunit of the adaptor protein complex 2 (AP-2), a heterotetrameric complex central to clathrin-mediated endocytosis. Related references: PMID:12694563, PMID:12952941, PMID:14745134
• The AP-2 complex functions as a cargo-selective adaptor in clathrin-coated vesicle formation, linking membrane proteins to the clathrin lattice. Related references: PMID:12694563, PMID:12952941, PMID:14745134
• AP2M1 recognizes Y-X-X-Φ sorting motifs in the cytoplasmic tails of transmembrane cargo; its cargo-binding site becomes accessible upon phosphorylation at Thr-156, a process facilitated by clathrin-activated AAK1 kinase. Related references: PMID:11877457, PMID:16581796
• In neurons, AP-2 participates in synaptic vesicle recycling and mediates the endocytosis of ADAM10 during long-term potentiation, highlighting its role in synaptic plasticity. Related references: PMID:12694563, PMID:12952941, PMID:14745134, PMID:23676497
• AP-2 also maintains normal post-endocytic trafficking through an ARF6-regulated, non-clathrin pathway, expanding its functional repertoire beyond coated vesicle formation. Related references: PMID:19033387
• At the protein level, AP2M1 is prominently expressed in the brain.
• Mutations or dysregulation of AP2M1 are linked to neurological disorders, including epilepsy and intellectual disability, underscoring its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen spans residues 134–433 of human AP2M1, covering a large region of the mu homology domain; sequence alignment with mouse and rat orthologs is recommended to assess epitope conservation.
• For Western blotting, a predominant band near 50 kDa (calculated molecular weight 49.7 kDa) is anticipated; brain tissue lysates, where AP2M1 is enriched, may serve as a suitable positive control.
• ELISA applications may require optimization of coating concentrations and blocking conditions when using recombinant protein or cell/tissue extracts.
• Since AP2M1 undergoes phosphorylation-dependent conformational changes, consider evaluating both phosphorylated and non-phosphorylated forms in relevant cellular contexts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering validated antibodies, ancillary reagents, and rare target sourcing for diagnostic and research applications. This anti-AP2M1 polyclonal antibody supports studies into clathrin-mediated endocytosis and neurological disease models, complementing our portfolio of adaptor protein and membrane trafficking reagents. Whether you require optimized antibody pairs, bulk-scale production, or custom sourcing solutions, CamelBio serves as a reliable partner from concept to clinic.