Anti-AP2M1 Monoclonal Antibody for WB and ELISA - Q96CW1

Core Product Specifications and Parameters

Biological Background: AP2M1 Function and Localization

• AP2M1 encodes the medium subunit of the clathrin adaptor AP-2 complex, which is essential for clathrin-dependent endocytosis at the plasma membrane. Related references: PMID:12694563, PMID:12952941, PMID:14745134
• The AP-2 complex functions as a cargo receptor by directly binding to tyrosine-based (Y-X-X-Φ) and dileucine-based sorting signals in the cytoplasmic tails of transmembrane proteins.
• Phosphorylation of the mu subunit at Thr-156 by the kinase AAK1 induces a conformational change that exposes the Y-X-X-Φ binding site, enabling cargo recognition at the membrane. Related references: PMID:11877457
• AP2M1 participates in the endocytosis of specific receptors, including ADAM10 during long-term potentiation in neurons. Related references: PMID:23676497
• It also plays a role in non-clathrin, ARF6-regulated post-endocytic trafficking. Related references: PMID:19033387
• Subcellularly, AP2M1 localizes to the inner leaflet of the plasma membrane at clathrin-coated pits and vesicles.
• The protein is highly expressed in brain tissue, consistent with its key role in synaptic vesicle recycling.
• AP2M1 is associated with disease variants linked to epilepsy and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 300-400) that is conserved across human, mouse, and rat, supporting cross-reactivity; verify in your specific samples.
• For Western blot, a band at ~50kDa is expected; include appropriate controls and optimize detection conditions.
• Consider using brain tissue or neuronal cell lines for relevant biological studies, given the high brain expression.
• As a phosphoprotein, include phosphatase inhibitors during lysis to preserve phosphorylation states.
• For immunofluorescence, permeabilization is necessary; expect punctate staining at the plasma membrane and coated pits.
• This antibody has not been validated for applications other than WB and ELISA; performance should be checked if used otherwise.

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