Anti-AP2A2/Alpha2-adaptin Monoclonal Antibody for WB, ELISA - O94973

Core Product Specifications and Parameters

Biological Background: Alpha2-adaptin/AP2A2 Function and Localization

• The AP2A2 gene encodes alpha-adaptin C, the alpha-2 subunit of the heterotetrameric adaptor protein complex 2 (AP-2), which is essential for clathrin-mediated endocytosis.
• AP-2 functions as a major clathrin adaptor, linking the clathrin lattice to both cargo proteins and phosphatidylinositol lipids at the plasma membrane, thereby driving the formation of clathrin-coated vesicles.
• The complex selectively recognizes Y-X-X-Φ and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo, enabling efficient sorting of membrane proteins for internalization.
• During long-term potentiation in hippocampal neurons, AP-2 mediates the endocytosis of ADAM10, a crucial step for synaptic plasticity. Related references: PMID:23676497
• The alpha-2 subunit binds polyphosphoinositide-containing lipids to position AP-2 on the membrane, while its C-terminal appendage domain serves as a scaffolding platform for endocytic accessory proteins.
• AP2A2 is expressed at the protein level in the brain, highlighting its significance in neuronal membrane trafficking.
• Subcellular localization is predominantly at the cell membrane and in coated pits, consistent with its role in early endocytic events.
• Keywords associated with AP2A2 include Alternative splicing, Coated pit, Endocytosis, Lipid-binding, Membrane, and Protein transport.

Experimental Guidance and Technical Tips

• The immunogen spans residues 590–939 of human AP2A2, a C-terminal region encompassing the functionally critical appendage domain. This region is highly conserved between human and mouse, supporting the observed cross-reactivity.
• For Western blotting, consider using reducing and denaturing conditions. The predicted molecular weight is 104 kDa; however, post-translational modifications or splice variants may cause slight band shifts. Validate loading controls and optimize blocking conditions.
• In ELISA applications, the antibody can be used for either capture or detection. Pre-test serial dilutions of the antibody and antigen coating concentrations to determine the optimal signal-to-noise ratio.
• When analyzing mouse samples, note that while the epitope region is conserved, always confirm species-specific reactivity in your assay system, as antibody performance may vary depending on sample preparation and detection protocols.

CamelBio: Your One-Stop Sourcing Bridge

As an IVD raw-material partner, CamelBio offers one-stop access to validated antibodies, antibody pairs, and bulk reagents like this anti-AP2A2 monoclonal antibody. Supporting research in endocytosis, neurobiology, and cell signaling, we help diagnostic manufacturers and labs accelerate development with optimized reagents—from concept to clinic. Whether you need custom antibody sourcing or rare target raw materials, CamelBio is your reliable bridge to high-quality IVD components.