Anti-AP2A1 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - O95782

Core Product Specifications and Parameters

Biological Background: AP2A1 Function and Localization

• AP2A1 encodes the alpha-1 subunit of the adaptor protein complex AP-2, a key component in clathrin-mediated endocytosis that bridges cargo selection and vesicle formation at the plasma membrane.
• The AP-2 complex binds directly to clathrin and membrane phosphoinositides, functioning as a major clathrin adaptor to drive the assembly of clathrin-coated vesicles (CCVs) for receptor-mediated endocytosis.
• AP2A1 recognizes sorting signals, including YXXΦ and [ED]XXXL[LI] motifs, within the cytoplasmic tails of transmembrane cargo proteins, enabling selective cargo recruitment into nascent vesicles.
• During long-term potentiation in hippocampal neurons, AP-2 mediates the endocytosis of ADAM10, a process critical for synaptic plasticity and memory formation. Related references: PMID:23676497
• The AP2A1 alpha subunit serves as a scaffolding platform via its C-terminal appendage domain, recruiting accessory proteins essential for endocytic regulation and cargo sorting.
• Subcellularly, AP2A1 localizes to the cell membrane and clathrin-coated pits, consistent with its role in initiating endocytic vesicle formation.
• AP2A1 is expressed as multiple isoforms through alternative splicing; isoform A is enriched in forebrain, skeletal muscle, and heart, while isoform B shows broad tissue distribution and is found in breast and prostate cancers.
• The protein is subject to phosphorylation, which may regulate its activity or interactions in trafficking pathways.

Experimental Guidance and Technical Tips

• When performing Western blot, expect a band near 108 kDa; we recommend using whole cell lysates from human, mouse, or rat cell lines as positive controls.
• For immunofluorescence, optimize fixation and permeabilization conditions to detect AP2A1 at the plasma membrane and in coated pits; co-staining with clathrin can confirm localization.
• The antibody may be used for ELISA; validate coating buffer, antigen concentration, and detection system empirically to achieve optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with streamlined access to IVD raw materials such as this anti-AP2A1 rabbit monoclonal antibody, enabling advanced study of clathrin-mediated endocytosis and membrane trafficking. Our comprehensive portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing services for rare targets—all designed to accelerate your workflow from concept to clinic.